Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10190 and BPHYT_RS10195 are separated by 76 nucleotides BPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotides BPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotides
BPHYT_RS10190: BPHYT_RS10190 - phosphonate monoester hydrolase, at 2,319,705 to 2,321,276
_RS10190
BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690
_RS10195
BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561
_RS10200
BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344
_RS10205
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2320.377 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.428 kb on + strand, within BPHYT_RS10190 at 2320.755 kb on + strand, within BPHYT_RS10190 at 2320.941 kb on + strand, within BPHYT_RS10190 at 2320.942 kb on - strand, within BPHYT_RS10190 at 2321.237 kb on + strand at 2321.437 kb on + strand at 2321.438 kb on - strand at 2321.467 kb on - strand at 2321.467 kb on - strand at 2322.023 kb on + strand, within BPHYT_RS10195 at 2322.023 kb on + strand, within BPHYT_RS10195 at 2322.412 kb on + strand, within BPHYT_RS10195 at 2322.451 kb on + strand, within BPHYT_RS10195 at 2322.568 kb on + strand at 2322.597 kb on - strand at 2322.617 kb on + strand at 2322.857 kb on - strand at 2322.930 kb on - strand at 2322.954 kb on - strand at 2323.118 kb on + strand at 2323.119 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,320,377 + BPHYT_RS10190 0.43 -1.9 2,320,398 + BPHYT_RS10190 0.44 -0.2 2,320,398 + BPHYT_RS10190 0.44 +0.6 2,320,428 + BPHYT_RS10190 0.46 +1.0 2,320,755 + BPHYT_RS10190 0.67 -1.9 2,320,941 + BPHYT_RS10190 0.79 +0.4 2,320,942 - BPHYT_RS10190 0.79 -0.6 2,321,237 + +3.6 2,321,437 + +1.2 2,321,438 - -1.7 2,321,467 - -0.4 2,321,467 - +0.6 2,322,023 + BPHYT_RS10195 0.50 -0.4 2,322,023 + BPHYT_RS10195 0.50 -0.1 2,322,412 + BPHYT_RS10195 0.79 -1.1 2,322,451 + BPHYT_RS10195 0.82 -1.6 2,322,568 + -0.7 2,322,597 - -0.0 2,322,617 + +0.2 2,322,857 - -0.6 2,322,930 - -1.0 2,322,954 - -0.1 2,323,118 + -0.2 2,323,119 - -0.5
Or see this region's nucleotide sequence