Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09780 and BPHYT_RS09785 overlap by 4 nucleotides BPHYT_RS09785 and BPHYT_RS09790 are separated by 116 nucleotides
BPHYT_RS09780: BPHYT_RS09780 - polysaccharide biosynthesis protein, at 2,219,973 to 2,221,439
_RS09780
BPHYT_RS09785: BPHYT_RS09785 - glycosyl transferase family 1, at 2,221,436 to 2,223,892
_RS09785
BPHYT_RS09790: BPHYT_RS09790 - acyltransferase, at 2,224,009 to 2,225,220
_RS09790
Position (kb)
2221
2222
2223
2224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2220.438 kb on + strand, within BPHYT_RS09780 at 2220.750 kb on + strand, within BPHYT_RS09780 at 2221.599 kb on - strand at 2221.764 kb on - strand, within BPHYT_RS09785 at 2221.764 kb on - strand, within BPHYT_RS09785 at 2221.811 kb on + strand, within BPHYT_RS09785 at 2221.886 kb on + strand, within BPHYT_RS09785 at 2221.887 kb on - strand, within BPHYT_RS09785 at 2221.932 kb on - strand, within BPHYT_RS09785 at 2222.084 kb on + strand, within BPHYT_RS09785 at 2222.084 kb on + strand, within BPHYT_RS09785 at 2222.191 kb on + strand, within BPHYT_RS09785 at 2222.693 kb on + strand, within BPHYT_RS09785 at 2222.704 kb on + strand, within BPHYT_RS09785 at 2223.467 kb on + strand, within BPHYT_RS09785 at 2223.468 kb on - strand, within BPHYT_RS09785 at 2223.836 kb on + strand at 2223.836 kb on + strand at 2223.837 kb on - strand at 2223.891 kb on - strand at 2223.985 kb on - strand at 2224.091 kb on + strand at 2224.091 kb on + strand at 2224.091 kb on + strand at 2224.092 kb on - strand at 2224.116 kb on + strand at 2224.117 kb on - strand at 2224.205 kb on + strand, within BPHYT_RS09790 at 2224.295 kb on + strand, within BPHYT_RS09790 at 2224.340 kb on + strand, within BPHYT_RS09790 at 2224.520 kb on + strand, within BPHYT_RS09790 at 2224.520 kb on + strand, within BPHYT_RS09790 at 2224.521 kb on - strand, within BPHYT_RS09790 at 2224.859 kb on + strand, within BPHYT_RS09790
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 2,220,438 + BPHYT_RS09780 0.32 +2.3 2,220,750 + BPHYT_RS09780 0.53 -0.4 2,221,599 - -0.7 2,221,764 - BPHYT_RS09785 0.13 -1.2 2,221,764 - BPHYT_RS09785 0.13 -0.8 2,221,811 + BPHYT_RS09785 0.15 +0.6 2,221,886 + BPHYT_RS09785 0.18 -0.5 2,221,887 - BPHYT_RS09785 0.18 -0.1 2,221,932 - BPHYT_RS09785 0.20 -0.7 2,222,084 + BPHYT_RS09785 0.26 -0.6 2,222,084 + BPHYT_RS09785 0.26 +0.9 2,222,191 + BPHYT_RS09785 0.31 -0.3 2,222,693 + BPHYT_RS09785 0.51 -2.0 2,222,704 + BPHYT_RS09785 0.52 +1.9 2,223,467 + BPHYT_RS09785 0.83 -1.7 2,223,468 - BPHYT_RS09785 0.83 +0.7 2,223,836 + +1.9 2,223,836 + -0.4 2,223,837 - -0.2 2,223,891 - +0.8 2,223,985 - +2.2 2,224,091 + -1.6 2,224,091 + -0.3 2,224,091 + +0.1 2,224,092 - -0.4 2,224,116 + -1.2 2,224,117 - +0.4 2,224,205 + BPHYT_RS09790 0.16 +1.3 2,224,295 + BPHYT_RS09790 0.24 +0.3 2,224,340 + BPHYT_RS09790 0.27 +1.5 2,224,520 + BPHYT_RS09790 0.42 +0.6 2,224,520 + BPHYT_RS09790 0.42 +0.4 2,224,521 - BPHYT_RS09790 0.42 +0.5 2,224,859 + BPHYT_RS09790 0.70 -1.3
Or see this region's nucleotide sequence