Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06750 and BPHYT_RS06755 are separated by 11 nucleotides BPHYT_RS06755 and BPHYT_RS06760 overlap by 4 nucleotides BPHYT_RS06760 and BPHYT_RS06765 are separated by 126 nucleotides BPHYT_RS06765 and BPHYT_RS06770 are separated by 106 nucleotides
BPHYT_RS06750: BPHYT_RS06750 - fructose-2,6-bisphosphatase, at 1,527,827 to 1,528,543
_RS06750
BPHYT_RS06755: BPHYT_RS06755 - enoyl-CoA hydratase, at 1,528,555 to 1,529,343
_RS06755
BPHYT_RS06760: BPHYT_RS06760 - 3-hydroxyacyl-CoA dehydrogenase, at 1,529,340 to 1,530,905
_RS06760
BPHYT_RS06765: BPHYT_RS06765 - glutathione S-transferase, at 1,531,032 to 1,531,679
_RS06765
BPHYT_RS06770: BPHYT_RS06770 - aminotransferase, at 1,531,786 to 1,532,958
_RS06770
Position (kb) 1529
1530
1531 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1528.479 kb on + strand at 1528.479 kb on + strand at 1528.483 kb on + strand at 1528.483 kb on + strand at 1528.533 kb on + strand at 1528.534 kb on - strand at 1529.240 kb on + strand, within BPHYT_RS06755 at 1530.136 kb on + strand, within BPHYT_RS06760 at 1530.136 kb on + strand, within BPHYT_RS06760 at 1530.149 kb on - strand, within BPHYT_RS06760 at 1530.299 kb on + strand, within BPHYT_RS06760 at 1530.300 kb on - strand, within BPHYT_RS06760 at 1530.300 kb on - strand, within BPHYT_RS06760 at 1530.341 kb on + strand, within BPHYT_RS06760 at 1530.539 kb on + strand, within BPHYT_RS06760 at 1530.539 kb on + strand, within BPHYT_RS06760 at 1530.539 kb on + strand, within BPHYT_RS06760 at 1530.539 kb on + strand, within BPHYT_RS06760 at 1530.679 kb on - strand, within BPHYT_RS06760 at 1530.679 kb on - strand, within BPHYT_RS06760 at 1530.796 kb on + strand at 1530.928 kb on - strand at 1530.928 kb on - strand at 1531.007 kb on - strand at 1531.007 kb on - strand at 1531.007 kb on - strand at 1531.068 kb on + strand at 1531.068 kb on + strand at 1531.069 kb on - strand at 1531.069 kb on - strand at 1531.233 kb on + strand, within BPHYT_RS06765 at 1531.354 kb on - strand, within BPHYT_RS06765 at 1531.465 kb on - strand, within BPHYT_RS06765 at 1531.506 kb on + strand, within BPHYT_RS06765 at 1531.506 kb on + strand, within BPHYT_RS06765 at 1531.507 kb on - strand, within BPHYT_RS06765 at 1531.507 kb on - strand, within BPHYT_RS06765 at 1531.548 kb on + strand, within BPHYT_RS06765 at 1531.549 kb on - strand, within BPHYT_RS06765 at 1531.549 kb on - strand at 1531.593 kb on + strand, within BPHYT_RS06765 at 1531.593 kb on + strand, within BPHYT_RS06765 at 1531.594 kb on - strand, within BPHYT_RS06765 at 1531.653 kb on + strand at 1531.653 kb on + strand at 1531.653 kb on + strand at 1531.653 kb on + strand at 1531.654 kb on - strand at 1531.654 kb on - strand at 1531.655 kb on + strand at 1531.656 kb on - strand at 1531.787 kb on + strand at 1531.788 kb on - strand at 1531.880 kb on + strand at 1531.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 1,528,479 + +0.6 1,528,479 + +0.2 1,528,483 + -0.7 1,528,483 + -1.2 1,528,533 + +1.3 1,528,534 - -1.3 1,529,240 + BPHYT_RS06755 0.87 +0.3 1,530,136 + BPHYT_RS06760 0.51 +1.6 1,530,136 + BPHYT_RS06760 0.51 +0.3 1,530,149 - BPHYT_RS06760 0.52 +0.2 1,530,299 + BPHYT_RS06760 0.61 +0.9 1,530,300 - BPHYT_RS06760 0.61 +0.7 1,530,300 - BPHYT_RS06760 0.61 +0.8 1,530,341 + BPHYT_RS06760 0.64 +2.9 1,530,539 + BPHYT_RS06760 0.77 +0.3 1,530,539 + BPHYT_RS06760 0.77 +1.2 1,530,539 + BPHYT_RS06760 0.77 +0.6 1,530,539 + BPHYT_RS06760 0.77 +1.8 1,530,679 - BPHYT_RS06760 0.86 +0.2 1,530,679 - BPHYT_RS06760 0.86 +0.9 1,530,796 + -0.7 1,530,928 - -1.2 1,530,928 - -0.3 1,531,007 - +0.4 1,531,007 - -0.4 1,531,007 - +0.3 1,531,068 + +0.2 1,531,068 + -0.2 1,531,069 - +0.4 1,531,069 - +0.5 1,531,233 + BPHYT_RS06765 0.31 +0.6 1,531,354 - BPHYT_RS06765 0.50 +0.1 1,531,465 - BPHYT_RS06765 0.67 -0.6 1,531,506 + BPHYT_RS06765 0.73 +0.4 1,531,506 + BPHYT_RS06765 0.73 -0.6 1,531,507 - BPHYT_RS06765 0.73 -1.6 1,531,507 - BPHYT_RS06765 0.73 -0.8 1,531,548 + BPHYT_RS06765 0.80 -0.4 1,531,549 - BPHYT_RS06765 0.80 +0.7 1,531,549 - +1.6 1,531,593 + BPHYT_RS06765 0.87 -0.2 1,531,593 + BPHYT_RS06765 0.87 +0.2 1,531,594 - BPHYT_RS06765 0.87 +0.6 1,531,653 + -2.2 1,531,653 + -2.0 1,531,653 + -1.1 1,531,653 + +0.2 1,531,654 - -1.0 1,531,654 - +0.2 1,531,655 + -0.3 1,531,656 - -0.7 1,531,787 + -0.1 1,531,788 - +0.2 1,531,880 + +1.8 1,531,881 - -0.6
Or see this region's nucleotide sequence