Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04135 and BPHYT_RS04140 are separated by 200 nucleotides BPHYT_RS04140 and BPHYT_RS04145 are separated by 117 nucleotides
BPHYT_RS04135: BPHYT_RS04135 - glycosyl transferase family 1, at 947,236 to 948,408
_RS04135
BPHYT_RS04140: BPHYT_RS04140 - polysaccharide biosynthesis protein, at 948,609 to 949,868
_RS04140
BPHYT_RS04145: BPHYT_RS04145 - family 2 glycosyl transferase, at 949,986 to 950,957
_RS04145
Position (kb) 948
949
950 Strain fitness (log2 ratio) -1
0
1
2
3 at 948.037 kb on + strand, within BPHYT_RS04135 at 948.192 kb on - strand, within BPHYT_RS04135 at 948.391 kb on - strand at 948.419 kb on - strand at 948.587 kb on + strand at 948.732 kb on + strand at 949.174 kb on + strand, within BPHYT_RS04140 at 949.287 kb on + strand, within BPHYT_RS04140 at 949.682 kb on + strand, within BPHYT_RS04140 at 949.946 kb on + strand at 949.947 kb on - strand at 950.026 kb on - strand at 950.182 kb on - strand, within BPHYT_RS04145 at 950.228 kb on + strand, within BPHYT_RS04145 at 950.228 kb on + strand, within BPHYT_RS04145 at 950.310 kb on - strand, within BPHYT_RS04145 at 950.310 kb on - strand, within BPHYT_RS04145 at 950.372 kb on - strand, within BPHYT_RS04145 at 950.465 kb on + strand, within BPHYT_RS04145 at 950.466 kb on - strand, within BPHYT_RS04145 at 950.687 kb on - strand, within BPHYT_RS04145 at 950.832 kb on - strand, within BPHYT_RS04145 at 950.838 kb on - strand, within BPHYT_RS04145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 948,037 + BPHYT_RS04135 0.68 -0.1 948,192 - BPHYT_RS04135 0.82 -0.5 948,391 - +0.7 948,419 - -0.5 948,587 + -1.2 948,732 + +3.7 949,174 + BPHYT_RS04140 0.45 -0.1 949,287 + BPHYT_RS04140 0.54 +1.1 949,682 + BPHYT_RS04140 0.85 +1.0 949,946 + -0.3 949,947 - -0.2 950,026 - -1.2 950,182 - BPHYT_RS04145 0.20 +0.1 950,228 + BPHYT_RS04145 0.25 +0.8 950,228 + BPHYT_RS04145 0.25 -0.5 950,310 - BPHYT_RS04145 0.33 -0.8 950,310 - BPHYT_RS04145 0.33 -0.5 950,372 - BPHYT_RS04145 0.40 -0.1 950,465 + BPHYT_RS04145 0.49 -0.8 950,466 - BPHYT_RS04145 0.49 -0.4 950,687 - BPHYT_RS04145 0.72 -0.6 950,832 - BPHYT_RS04145 0.87 -0.5 950,838 - BPHYT_RS04145 0.88 -0.5
Or see this region's nucleotide sequence