Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01030 and BPHYT_RS01035 are separated by 341 nucleotides BPHYT_RS01035 and BPHYT_RS01040 overlap by 4 nucleotides BPHYT_RS01040 and BPHYT_RS01045 are separated by 192 nucleotides
BPHYT_RS01030: BPHYT_RS01030 - bacterioferritin, at 237,119 to 237,598
_RS01030
BPHYT_RS01035: BPHYT_RS01035 - cyanate hydratase, at 237,940 to 238,389
_RS01035
BPHYT_RS01040: BPHYT_RS01040 - Fis family transcriptional regulator, at 238,386 to 238,841
_RS01040
BPHYT_RS01045: BPHYT_RS01045 - hypothetical protein, at 239,034 to 239,327
_RS01045
Position (kb) 237
238
239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 237.025 kb on + strand at 237.025 kb on + strand at 237.025 kb on + strand at 237.026 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.120 kb on + strand at 237.153 kb on + strand at 237.279 kb on + strand, within BPHYT_RS01030 at 237.466 kb on - strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.505 kb on - strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.627 kb on - strand at 237.702 kb on + strand at 237.810 kb on + strand at 237.810 kb on + strand at 237.841 kb on + strand at 237.879 kb on - strand at 237.889 kb on + strand at 237.889 kb on + strand at 237.890 kb on - strand at 238.204 kb on + strand, within BPHYT_RS01035 at 238.204 kb on + strand, within BPHYT_RS01035 at 238.376 kb on - strand at 238.384 kb on + strand at 238.385 kb on - strand at 238.388 kb on - strand at 238.657 kb on + strand, within BPHYT_RS01040 at 238.711 kb on + strand, within BPHYT_RS01040 at 238.712 kb on - strand, within BPHYT_RS01040 at 238.844 kb on + strand at 238.845 kb on - strand at 238.845 kb on - strand at 238.845 kb on - strand at 238.849 kb on - strand at 238.849 kb on - strand at 238.895 kb on + strand at 238.899 kb on + strand at 238.900 kb on - strand at 238.904 kb on + strand at 238.905 kb on - strand at 238.905 kb on - strand at 238.912 kb on - strand at 238.980 kb on + strand at 238.980 kb on + strand at 238.995 kb on + strand at 238.996 kb on - strand at 239.212 kb on + strand, within BPHYT_RS01045 at 239.212 kb on + strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.296 kb on + strand, within BPHYT_RS01045 at 239.302 kb on + strand at 239.302 kb on + strand at 239.302 kb on + strand at 239.303 kb on - strand at 239.303 kb on - strand at 239.325 kb on + strand at 239.339 kb on + strand at 239.339 kb on + strand at 239.340 kb on - strand at 239.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 237,025 + -0.4 237,025 + -0.2 237,025 + +0.1 237,026 - +0.2 237,030 - -0.1 237,030 - +0.6 237,030 - -1.3 237,030 - +1.3 237,030 - -0.3 237,030 - +0.1 237,120 + +1.1 237,153 + -1.1 237,279 + BPHYT_RS01030 0.33 -0.3 237,466 - BPHYT_RS01030 0.72 +0.1 237,504 + BPHYT_RS01030 0.80 +0.8 237,504 + BPHYT_RS01030 0.80 +0.6 237,505 - BPHYT_RS01030 0.80 +1.0 237,525 + BPHYT_RS01030 0.85 +0.3 237,525 + BPHYT_RS01030 0.85 -0.4 237,627 - -0.2 237,702 + -3.1 237,810 + +0.5 237,810 + +0.7 237,841 + -0.5 237,879 - +0.7 237,889 + +0.1 237,889 + -0.3 237,890 - +0.5 238,204 + BPHYT_RS01035 0.59 +0.1 238,204 + BPHYT_RS01035 0.59 +0.2 238,376 - -0.1 238,384 + -0.9 238,385 - -0.4 238,388 - -0.5 238,657 + BPHYT_RS01040 0.59 +0.0 238,711 + BPHYT_RS01040 0.71 -1.0 238,712 - BPHYT_RS01040 0.71 -1.5 238,844 + +0.3 238,845 - -1.1 238,845 - +0.2 238,845 - +0.0 238,849 - -0.5 238,849 - -0.0 238,895 + +0.5 238,899 + -0.1 238,900 - -0.2 238,904 + -0.5 238,905 - +1.7 238,905 - +0.3 238,912 - -0.0 238,980 + +0.0 238,980 + -0.8 238,995 + -0.0 238,996 - -0.3 239,212 + BPHYT_RS01045 0.61 +0.8 239,212 + BPHYT_RS01045 0.61 -0.3 239,213 - BPHYT_RS01045 0.61 +0.1 239,213 - BPHYT_RS01045 0.61 +0.5 239,213 - BPHYT_RS01045 0.61 -0.5 239,213 - BPHYT_RS01045 0.61 +0.4 239,296 + BPHYT_RS01045 0.89 +1.5 239,302 + -0.3 239,302 + +0.2 239,302 + -0.1 239,303 - +1.9 239,303 - +0.5 239,325 + +0.5 239,339 + +0.3 239,339 + +0.0 239,340 - -1.0 239,340 - -0.7
Or see this region's nucleotide sequence