Experiment: 36hr PHYTO8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01025 and BPHYT_RS01030 are separated by 366 nucleotides BPHYT_RS01030 and BPHYT_RS01035 are separated by 341 nucleotides BPHYT_RS01035 and BPHYT_RS01040 overlap by 4 nucleotides
BPHYT_RS01025: BPHYT_RS01025 - hypothetical protein, at 236,189 to 236,752
_RS01025
BPHYT_RS01030: BPHYT_RS01030 - bacterioferritin, at 237,119 to 237,598
_RS01030
BPHYT_RS01035: BPHYT_RS01035 - cyanate hydratase, at 237,940 to 238,389
_RS01035
BPHYT_RS01040: BPHYT_RS01040 - Fis family transcriptional regulator, at 238,386 to 238,841
_RS01040
Position (kb) 237
238 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 236.155 kb on + strand at 236.155 kb on + strand at 236.160 kb on - strand at 236.213 kb on - strand at 237.025 kb on + strand at 237.025 kb on + strand at 237.025 kb on + strand at 237.026 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.030 kb on - strand at 237.120 kb on + strand at 237.153 kb on + strand at 237.279 kb on + strand, within BPHYT_RS01030 at 237.466 kb on - strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.504 kb on + strand, within BPHYT_RS01030 at 237.505 kb on - strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.525 kb on + strand, within BPHYT_RS01030 at 237.627 kb on - strand at 237.702 kb on + strand at 237.810 kb on + strand at 237.810 kb on + strand at 237.841 kb on + strand at 237.879 kb on - strand at 237.889 kb on + strand at 237.889 kb on + strand at 237.890 kb on - strand at 238.204 kb on + strand, within BPHYT_RS01035 at 238.204 kb on + strand, within BPHYT_RS01035 at 238.376 kb on - strand at 238.384 kb on + strand at 238.385 kb on - strand at 238.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO8 MOI 10 remove 236,155 + -0.9 236,155 + -1.1 236,160 - +1.2 236,213 - -1.1 237,025 + -0.4 237,025 + -0.2 237,025 + +0.1 237,026 - +0.2 237,030 - -0.1 237,030 - +0.6 237,030 - -1.3 237,030 - +1.3 237,030 - -0.3 237,030 - +0.1 237,120 + +1.1 237,153 + -1.1 237,279 + BPHYT_RS01030 0.33 -0.3 237,466 - BPHYT_RS01030 0.72 +0.1 237,504 + BPHYT_RS01030 0.80 +0.8 237,504 + BPHYT_RS01030 0.80 +0.6 237,505 - BPHYT_RS01030 0.80 +1.0 237,525 + BPHYT_RS01030 0.85 +0.3 237,525 + BPHYT_RS01030 0.85 -0.4 237,627 - -0.2 237,702 + -3.1 237,810 + +0.5 237,810 + +0.7 237,841 + -0.5 237,879 - +0.7 237,889 + +0.1 237,889 + -0.3 237,890 - +0.5 238,204 + BPHYT_RS01035 0.59 +0.1 238,204 + BPHYT_RS01035 0.59 +0.2 238,376 - -0.1 238,384 + -0.9 238,385 - -0.4 238,388 - -0.5
Or see this region's nucleotide sequence