Experiment: 36hr PHYTO7 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31115 and BPHYT_RS31120 are separated by 120 nucleotides BPHYT_RS31120 and BPHYT_RS31125 are separated by 71 nucleotides BPHYT_RS31125 and BPHYT_RS31130 are separated by 100 nucleotides
BPHYT_RS31115: BPHYT_RS31115 - glucosidase, at 2,535,852 to 2,538,593
_RS31115
BPHYT_RS31120: BPHYT_RS31120 - pseudouridine-5'-phosphate glycosidase, at 2,538,714 to 2,539,667
_RS31120
BPHYT_RS31125: BPHYT_RS31125 - hypothetical protein, at 2,539,739 to 2,540,188
_RS31125
BPHYT_RS31130: BPHYT_RS31130 - hypothetical protein, at 2,540,289 to 2,540,492
_RS31130
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2537.898 kb on - strand, within BPHYT_RS31115 at 2538.033 kb on - strand, within BPHYT_RS31115 at 2538.033 kb on - strand, within BPHYT_RS31115 at 2538.187 kb on + strand, within BPHYT_RS31115 at 2538.193 kb on + strand, within BPHYT_RS31115 at 2538.209 kb on - strand, within BPHYT_RS31115 at 2538.452 kb on - strand at 2538.458 kb on + strand at 2538.623 kb on + strand at 2538.624 kb on - strand at 2539.060 kb on + strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.699 kb on - strand at 2539.700 kb on + strand at 2539.700 kb on + strand at 2539.786 kb on + strand, within BPHYT_RS31125 at 2539.787 kb on - strand, within BPHYT_RS31125 at 2539.888 kb on - strand, within BPHYT_RS31125 at 2540.212 kb on - strand at 2540.328 kb on + strand, within BPHYT_RS31130 at 2540.433 kb on - strand, within BPHYT_RS31130 at 2540.433 kb on - strand, within BPHYT_RS31130 at 2540.434 kb on + strand, within BPHYT_RS31130 at 2540.492 kb on + strand at 2540.492 kb on + strand at 2540.560 kb on - strand at 2540.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1 remove 2,537,898 - BPHYT_RS31115 0.75 +0.1 2,538,033 - BPHYT_RS31115 0.80 -0.1 2,538,033 - BPHYT_RS31115 0.80 -1.4 2,538,187 + BPHYT_RS31115 0.85 +2.5 2,538,193 + BPHYT_RS31115 0.85 +0.1 2,538,209 - BPHYT_RS31115 0.86 -1.3 2,538,452 - +0.0 2,538,458 + +0.0 2,538,623 + -0.3 2,538,624 - -0.3 2,539,060 + BPHYT_RS31120 0.36 +1.0 2,539,061 - BPHYT_RS31120 0.36 -2.6 2,539,061 - BPHYT_RS31120 0.36 +0.3 2,539,061 - BPHYT_RS31120 0.36 -1.0 2,539,699 - +1.3 2,539,700 + -1.2 2,539,700 + +1.0 2,539,786 + BPHYT_RS31125 0.10 +0.2 2,539,787 - BPHYT_RS31125 0.11 -0.3 2,539,888 - BPHYT_RS31125 0.33 +1.1 2,540,212 - -0.2 2,540,328 + BPHYT_RS31130 0.19 +0.7 2,540,433 - BPHYT_RS31130 0.71 +0.6 2,540,433 - BPHYT_RS31130 0.71 +0.9 2,540,434 + BPHYT_RS31130 0.71 -0.2 2,540,492 + +0.8 2,540,492 + -0.6 2,540,560 - -0.7 2,540,597 + +1.0
Or see this region's nucleotide sequence