Experiment: 36hr PHYTO7 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30820 and BPHYT_RS30825 are separated by 334 nucleotides BPHYT_RS30825 and BPHYT_RS30830 are separated by 145 nucleotides BPHYT_RS30830 and BPHYT_RS30835 are separated by 82 nucleotides BPHYT_RS30835 and BPHYT_RS30840 are separated by 154 nucleotides
BPHYT_RS30820: BPHYT_RS30820 - GntR family transcriptional regulator, at 2,470,894 to 2,471,634
_RS30820
BPHYT_RS30825: BPHYT_RS30825 - extradiol dioxygenase, at 2,471,969 to 2,472,400
_RS30825
BPHYT_RS30830: BPHYT_RS30830 - MFS transporter, at 2,472,546 to 2,473,859
_RS30830
BPHYT_RS30835: BPHYT_RS30835 - hypothetical protein, at 2,473,942 to 2,474,304
_RS30835
BPHYT_RS30840: BPHYT_RS30840 - small mechanosensitive ion channel protein MscS, at 2,474,459 to 2,475,673
_RS30840
Position (kb)
2472
2473
2474 Strain fitness (log2 ratio)
-1
0
1 at 2471.786 kb on - strand at 2472.032 kb on + strand, within BPHYT_RS30825 at 2472.124 kb on + strand, within BPHYT_RS30825 at 2472.398 kb on + strand at 2472.431 kb on + strand at 2472.432 kb on - strand at 2472.592 kb on + strand at 2472.733 kb on - strand, within BPHYT_RS30830 at 2472.733 kb on - strand, within BPHYT_RS30830 at 2472.800 kb on - strand, within BPHYT_RS30830 at 2473.888 kb on + strand at 2473.889 kb on - strand at 2473.892 kb on + strand at 2473.943 kb on + strand at 2474.427 kb on + strand at 2474.481 kb on + strand at 2474.527 kb on - strand at 2474.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1 remove 2,471,786 - +0.1 2,472,032 + BPHYT_RS30825 0.15 -0.5 2,472,124 + BPHYT_RS30825 0.36 -1.0 2,472,398 + -0.7 2,472,431 + -1.5 2,472,432 - +0.3 2,472,592 + +0.7 2,472,733 - BPHYT_RS30830 0.14 +0.1 2,472,733 - BPHYT_RS30830 0.14 +1.5 2,472,800 - BPHYT_RS30830 0.19 +0.1 2,473,888 + -0.7 2,473,889 - -0.3 2,473,892 + -1.0 2,473,943 + +1.0 2,474,427 + -0.1 2,474,481 + -1.3 2,474,527 - +1.4 2,474,555 + -0.2
Or see this region's nucleotide sequence