Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28685

Experiment: 36hr PHYTO7 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28675 and BPHYT_RS28680 are separated by 120 nucleotidesBPHYT_RS28680 and BPHYT_RS28685 are separated by 28 nucleotidesBPHYT_RS28685 and BPHYT_RS28690 are separated by 65 nucleotides BPHYT_RS28675: BPHYT_RS28675 - hypothetical protein, at 1,986,879 to 1,987,544 _RS28675 BPHYT_RS28680: BPHYT_RS28680 - porin, at 1,987,665 to 1,988,180 _RS28680 BPHYT_RS28685: BPHYT_RS28685 - GntR family transcriptional regulator, at 1,988,209 to 1,989,660 _RS28685 BPHYT_RS28690: BPHYT_RS28690 - diguanylate phosphodiesterase, at 1,989,726 to 1,991,282 _RS28690 Position (kb) 1988 1989 1990Strain fitness (log2 ratio) -2 -1 0 1at 1987.772 kb on - strand, within BPHYT_RS28680at 1987.869 kb on + strand, within BPHYT_RS28680at 1988.972 kb on - strand, within BPHYT_RS28685at 1989.188 kb on + strand, within BPHYT_RS28685at 1989.189 kb on - strand, within BPHYT_RS28685at 1989.519 kb on - strandat 1989.842 kb on - strandat 1990.241 kb on - strand, within BPHYT_RS28690at 1990.614 kb on + strand, within BPHYT_RS28690

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1
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1,987,772 - BPHYT_RS28680 0.21 -1.5
1,987,869 + BPHYT_RS28680 0.40 +0.9
1,988,972 - BPHYT_RS28685 0.53 -2.3
1,989,188 + BPHYT_RS28685 0.67 -1.4
1,989,189 - BPHYT_RS28685 0.67 -0.4
1,989,519 - +0.6
1,989,842 - -0.6
1,990,241 - BPHYT_RS28690 0.33 -1.9
1,990,614 + BPHYT_RS28690 0.57 +0.5

Or see this region's nucleotide sequence