Experiment: 36hr PHYTO7 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21130 and BPHYT_RS21135 overlap by 4 nucleotides BPHYT_RS21135 and BPHYT_RS21140 are separated by 32 nucleotides BPHYT_RS21140 and BPHYT_RS21145 are separated by 143 nucleotides
BPHYT_RS21130: BPHYT_RS21130 - F420-dependent oxidoreductase, at 301,771 to 302,550
_RS21130
BPHYT_RS21135: BPHYT_RS21135 - NADH-ubiquinone oxidoreductase subunit 6, at 302,547 to 303,227
_RS21135
BPHYT_RS21140: BPHYT_RS21140 - methylenetetrahydromethanopterin reductase, at 303,260 to 304,261
_RS21140
BPHYT_RS21145: BPHYT_RS21145 - allantoate amidohydrolase, at 304,405 to 305,643
_RS21145
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 302.621 kb on - strand, within BPHYT_RS21135 at 302.692 kb on + strand, within BPHYT_RS21135 at 302.693 kb on - strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.698 kb on + strand, within BPHYT_RS21135 at 302.944 kb on + strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 302.945 kb on - strand, within BPHYT_RS21135 at 303.225 kb on + strand at 303.225 kb on + strand at 303.226 kb on - strand at 303.975 kb on - strand, within BPHYT_RS21140 at 304.038 kb on - strand, within BPHYT_RS21140 at 304.190 kb on + strand at 304.773 kb on + strand, within BPHYT_RS21145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1 remove 302,621 - BPHYT_RS21135 0.11 -0.0 302,692 + BPHYT_RS21135 0.21 -1.2 302,693 - BPHYT_RS21135 0.21 -1.3 302,698 + BPHYT_RS21135 0.22 +0.9 302,698 + BPHYT_RS21135 0.22 -1.3 302,944 + BPHYT_RS21135 0.58 +2.4 302,945 - BPHYT_RS21135 0.58 -1.8 302,945 - BPHYT_RS21135 0.58 -0.2 302,945 - BPHYT_RS21135 0.58 -1.0 303,225 + +0.1 303,225 + +1.4 303,226 - +3.2 303,975 - BPHYT_RS21140 0.71 +0.6 304,038 - BPHYT_RS21140 0.78 +1.4 304,190 + -1.6 304,773 + BPHYT_RS21145 0.30 -0.2
Or see this region's nucleotide sequence