Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05460

Experiment: 36hr PHYTO7 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05455 and BPHYT_RS05460 are separated by 96 nucleotidesBPHYT_RS05460 and BPHYT_RS05465 are separated by 570 nucleotides BPHYT_RS05455: BPHYT_RS05455 - AraC family transcriptional regulator, at 1,252,422 to 1,253,267 _RS05455 BPHYT_RS05460: BPHYT_RS05460 - chloroperoxidase, at 1,253,364 to 1,254,194 _RS05460 BPHYT_RS05465: BPHYT_RS05465 - hypothetical protein, at 1,254,765 to 1,256,864 _RS05465 Position (kb) 1253 1254 1255Strain fitness (log2 ratio) -2 -1 0 1at 1252.635 kb on + strand, within BPHYT_RS05455at 1252.635 kb on + strand, within BPHYT_RS05455at 1252.636 kb on - strand, within BPHYT_RS05455at 1252.766 kb on + strand, within BPHYT_RS05455at 1253.338 kb on - strandat 1253.605 kb on + strand, within BPHYT_RS05460at 1253.690 kb on - strand, within BPHYT_RS05460at 1253.722 kb on + strand, within BPHYT_RS05460at 1254.185 kb on + strandat 1254.352 kb on + strandat 1254.357 kb on - strandat 1254.357 kb on - strandat 1254.444 kb on - strandat 1254.613 kb on + strandat 1254.614 kb on - strandat 1254.652 kb on + strandat 1254.822 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1
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1,252,635 + BPHYT_RS05455 0.25 +0.2
1,252,635 + BPHYT_RS05455 0.25 -0.7
1,252,636 - BPHYT_RS05455 0.25 +1.3
1,252,766 + BPHYT_RS05455 0.41 +0.5
1,253,338 - -0.6
1,253,605 + BPHYT_RS05460 0.29 +1.3
1,253,690 - BPHYT_RS05460 0.39 -0.7
1,253,722 + BPHYT_RS05460 0.43 +1.1
1,254,185 + +0.5
1,254,352 + -1.0
1,254,357 - +0.9
1,254,357 - +0.1
1,254,444 - +0.3
1,254,613 + -0.5
1,254,614 - -2.3
1,254,652 + +1.0
1,254,822 + -0.0

Or see this region's nucleotide sequence