Experiment: 36hr PHYTO7 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02995 and BPHYT_RS03000 are separated by 10 nucleotides BPHYT_RS03000 and BPHYT_RS03005 overlap by 4 nucleotides
BPHYT_RS02995: BPHYT_RS02995 - 2-methylcitrate dehydratase, at 667,181 to 668,593
_RS02995
BPHYT_RS03000: BPHYT_RS03000 - acyl-CoA dehydrogenase, at 668,604 to 669,767
_RS03000
BPHYT_RS03005: BPHYT_RS03005 - CoA transferase, at 669,764 to 670,954
_RS03005
Position (kb) 668
669
670 Strain fitness (log2 ratio) -2
-1
0
1
2 at 668.210 kb on + strand, within BPHYT_RS02995 at 668.211 kb on - strand, within BPHYT_RS02995 at 668.211 kb on - strand, within BPHYT_RS02995 at 668.352 kb on - strand, within BPHYT_RS02995 at 668.468 kb on + strand at 668.526 kb on + strand at 668.527 kb on - strand at 668.591 kb on + strand at 668.592 kb on - strand at 668.717 kb on + strand at 668.817 kb on + strand, within BPHYT_RS03000 at 668.817 kb on + strand, within BPHYT_RS03000 at 668.933 kb on + strand, within BPHYT_RS03000 at 668.934 kb on - strand, within BPHYT_RS03000 at 668.937 kb on + strand, within BPHYT_RS03000 at 668.938 kb on - strand, within BPHYT_RS03000 at 669.052 kb on - strand, within BPHYT_RS03000 at 669.224 kb on + strand, within BPHYT_RS03000 at 669.225 kb on - strand, within BPHYT_RS03000 at 669.319 kb on + strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.406 kb on - strand, within BPHYT_RS03000 at 669.532 kb on - strand, within BPHYT_RS03000 at 669.532 kb on - strand, within BPHYT_RS03000 at 669.637 kb on - strand, within BPHYT_RS03000 at 669.654 kb on + strand at 669.655 kb on - strand at 669.693 kb on - strand at 669.693 kb on - strand at 670.095 kb on - strand, within BPHYT_RS03005 at 670.095 kb on - strand, within BPHYT_RS03005 at 670.148 kb on + strand, within BPHYT_RS03005 at 670.190 kb on + strand, within BPHYT_RS03005 at 670.191 kb on - strand, within BPHYT_RS03005 at 670.387 kb on + strand, within BPHYT_RS03005 at 670.388 kb on - strand, within BPHYT_RS03005 at 670.398 kb on - strand, within BPHYT_RS03005 at 670.413 kb on - strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1 remove 668,210 + BPHYT_RS02995 0.73 +0.1 668,211 - BPHYT_RS02995 0.73 -0.5 668,211 - BPHYT_RS02995 0.73 -0.5 668,352 - BPHYT_RS02995 0.83 -0.1 668,468 + -1.5 668,526 + +0.1 668,527 - +1.0 668,591 + -0.9 668,592 - -0.2 668,717 + +0.7 668,817 + BPHYT_RS03000 0.18 -0.4 668,817 + BPHYT_RS03000 0.18 -1.0 668,933 + BPHYT_RS03000 0.28 +0.1 668,934 - BPHYT_RS03000 0.28 -0.7 668,937 + BPHYT_RS03000 0.29 -2.3 668,938 - BPHYT_RS03000 0.29 +0.2 669,052 - BPHYT_RS03000 0.38 +0.8 669,224 + BPHYT_RS03000 0.53 +0.5 669,225 - BPHYT_RS03000 0.53 -0.7 669,319 + BPHYT_RS03000 0.61 +0.1 669,406 - BPHYT_RS03000 0.69 -0.4 669,406 - BPHYT_RS03000 0.69 -1.0 669,406 - BPHYT_RS03000 0.69 -1.8 669,532 - BPHYT_RS03000 0.80 +0.7 669,532 - BPHYT_RS03000 0.80 -0.2 669,637 - BPHYT_RS03000 0.89 -0.1 669,654 + -0.1 669,655 - +2.3 669,693 - +0.7 669,693 - +0.5 670,095 - BPHYT_RS03005 0.28 +0.7 670,095 - BPHYT_RS03005 0.28 +0.6 670,148 + BPHYT_RS03005 0.32 +0.0 670,190 + BPHYT_RS03005 0.36 +0.2 670,191 - BPHYT_RS03005 0.36 +0.2 670,387 + BPHYT_RS03005 0.52 -1.4 670,388 - BPHYT_RS03005 0.52 -0.1 670,398 - BPHYT_RS03005 0.53 -0.6 670,413 - BPHYT_RS03005 0.54 +0.8 670,484 + BPHYT_RS03005 0.60 +0.3 670,484 + BPHYT_RS03005 0.60 +0.2 670,484 + BPHYT_RS03005 0.60 -0.1
Or see this region's nucleotide sequence