Experiment: 36hr PHYTO7 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02760 and BPHYT_RS02765 are separated by 35 nucleotides BPHYT_RS02765 and BPHYT_RS02770 overlap by 11 nucleotides BPHYT_RS02770 and BPHYT_RS02775 are separated by 338 nucleotides
BPHYT_RS02760: BPHYT_RS02760 - cytochrome BD oxidase subunit II, at 619,886 to 621,022
_RS02760
BPHYT_RS02765: BPHYT_RS02765 - cytochrome BD oxidase subunit I, at 621,058 to 622,650
_RS02765
BPHYT_RS02770: BPHYT_RS02770 - membrane protein, at 622,640 to 622,900
_RS02770
BPHYT_RS02775: BPHYT_RS02775 - RNA polymerase sigma 70, at 623,239 to 624,174
_RS02775
Position (kb) 621
622
623 Strain fitness (log2 ratio) -2
-1
0
1
2 at 620.557 kb on - strand, within BPHYT_RS02760 at 620.677 kb on + strand, within BPHYT_RS02760 at 621.224 kb on + strand, within BPHYT_RS02765 at 622.163 kb on + strand, within BPHYT_RS02765 at 622.397 kb on + strand, within BPHYT_RS02765 at 622.526 kb on + strand at 622.526 kb on + strand at 622.526 kb on + strand at 622.527 kb on - strand at 622.726 kb on + strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.727 kb on - strand, within BPHYT_RS02770 at 622.778 kb on + strand, within BPHYT_RS02770 at 622.892 kb on + strand at 623.054 kb on + strand at 623.055 kb on - strand at 623.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 0.1 remove 620,557 - BPHYT_RS02760 0.59 -0.7 620,677 + BPHYT_RS02760 0.70 +0.6 621,224 + BPHYT_RS02765 0.10 -0.3 622,163 + BPHYT_RS02765 0.69 -0.5 622,397 + BPHYT_RS02765 0.84 -0.7 622,526 + -1.1 622,526 + -0.3 622,526 + +2.3 622,527 - +2.3 622,726 + BPHYT_RS02770 0.33 +1.0 622,727 - BPHYT_RS02770 0.33 +0.3 622,727 - BPHYT_RS02770 0.33 -1.5 622,727 - BPHYT_RS02770 0.33 -0.7 622,778 + BPHYT_RS02770 0.53 +1.6 622,892 + +0.3 623,054 + -0.0 623,055 - -0.0 623,055 - -2.4
Or see this region's nucleotide sequence