Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34835 and BPHYT_RS34840 are separated by 59 nucleotides BPHYT_RS34840 and BPHYT_RS34845 are separated by 137 nucleotides BPHYT_RS34845 and BPHYT_RS34850 are separated by 214 nucleotides BPHYT_RS34850 and BPHYT_RS34855 are separated by 118 nucleotides
BPHYT_RS34835: BPHYT_RS34835 - glycosyl transferase, at 3,420,515 to 3,421,687
_RS34835
BPHYT_RS34840: BPHYT_RS34840 - hypothetical protein, at 3,421,747 to 3,422,310
_RS34840
BPHYT_RS34845: BPHYT_RS34845 - NAD-dependent dehydratase, at 3,422,448 to 3,423,458
_RS34845
BPHYT_RS34850: BPHYT_RS34850 - acylphosphatase, at 3,423,673 to 3,423,966
_RS34850
BPHYT_RS34855: BPHYT_RS34855 - methyltransferase, at 3,424,085 to 3,425,065
_RS34855
Position (kb)
3422
3423
3424 Strain fitness (log2 ratio)
-1
0
1 at 3421.542 kb on - strand, within BPHYT_RS34835 at 3421.592 kb on + strand at 3421.593 kb on - strand at 3421.749 kb on - strand at 3421.766 kb on + strand at 3421.767 kb on - strand at 3421.767 kb on - strand at 3422.326 kb on + strand at 3422.354 kb on + strand at 3422.355 kb on - strand at 3422.363 kb on - strand at 3422.363 kb on - strand at 3422.373 kb on - strand at 3422.389 kb on - strand at 3422.435 kb on - strand at 3423.496 kb on - strand at 3423.584 kb on + strand at 3423.627 kb on + strand at 3423.628 kb on - strand at 3423.660 kb on + strand at 3423.710 kb on - strand, within BPHYT_RS34850 at 3423.710 kb on - strand, within BPHYT_RS34850 at 3423.964 kb on + strand at 3424.451 kb on + strand, within BPHYT_RS34855
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 3,421,542 - BPHYT_RS34835 0.88 +1.5 3,421,592 + -0.9 3,421,593 - -0.6 3,421,749 - -0.5 3,421,766 + +0.7 3,421,767 - -0.8 3,421,767 - +0.5 3,422,326 + -0.3 3,422,354 + +0.1 3,422,355 - -0.2 3,422,363 - +0.1 3,422,363 - -0.1 3,422,373 - -1.0 3,422,389 - +0.4 3,422,435 - +1.3 3,423,496 - +0.4 3,423,584 + +0.3 3,423,627 + +0.2 3,423,628 - -0.3 3,423,660 + -0.3 3,423,710 - BPHYT_RS34850 0.13 -0.3 3,423,710 - BPHYT_RS34850 0.13 -0.0 3,423,964 + -0.0 3,424,451 + BPHYT_RS34855 0.37 +1.3
Or see this region's nucleotide sequence