Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34640 and BPHYT_RS34645 are separated by 108 nucleotides BPHYT_RS34645 and BPHYT_RS34650 are separated by 97 nucleotides BPHYT_RS34650 and BPHYT_RS34655 are separated by 13 nucleotides
BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946
_RS34640
BPHYT_RS34645: BPHYT_RS34645 - hypothetical protein, at 3,380,055 to 3,380,705
_RS34645
BPHYT_RS34650: BPHYT_RS34650 - l-threonine 3-dehydrogenase, at 3,380,803 to 3,381,834
_RS34650
BPHYT_RS34655: BPHYT_RS34655 - 2-amino-3-ketobutyrate CoA ligase, at 3,381,848 to 3,383,047
_RS34655
Position (kb)
3380
3381
3382 Strain fitness (log2 ratio)
-2
-1
0
1 at 3379.865 kb on - strand, within BPHYT_RS34640 at 3379.948 kb on + strand at 3379.949 kb on - strand at 3380.019 kb on + strand at 3380.020 kb on - strand at 3380.020 kb on - strand at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.154 kb on - strand, within BPHYT_RS34645 at 3380.247 kb on + strand, within BPHYT_RS34645 at 3380.248 kb on - strand, within BPHYT_RS34645 at 3380.635 kb on - strand, within BPHYT_RS34645 at 3380.654 kb on + strand at 3380.800 kb on - strand at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.094 kb on - strand, within BPHYT_RS34650 at 3381.240 kb on - strand, within BPHYT_RS34650 at 3381.642 kb on - strand, within BPHYT_RS34650 at 3381.712 kb on - strand, within BPHYT_RS34650 at 3381.853 kb on + strand at 3381.854 kb on - strand at 3382.236 kb on + strand, within BPHYT_RS34655 at 3382.780 kb on + strand, within BPHYT_RS34655
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 3,379,865 - BPHYT_RS34640 0.88 -0.2 3,379,948 + +0.1 3,379,949 - +0.8 3,380,019 + +0.3 3,380,020 - -0.8 3,380,020 - -1.1 3,380,148 - BPHYT_RS34645 0.14 -2.0 3,380,148 - BPHYT_RS34645 0.14 -0.1 3,380,154 - BPHYT_RS34645 0.15 +0.6 3,380,247 + BPHYT_RS34645 0.29 +0.7 3,380,248 - BPHYT_RS34645 0.30 -0.8 3,380,635 - BPHYT_RS34645 0.89 -0.6 3,380,654 + -0.2 3,380,800 - +0.9 3,381,094 - BPHYT_RS34650 0.28 +1.7 3,381,094 - BPHYT_RS34650 0.28 -1.5 3,381,240 - BPHYT_RS34650 0.42 -2.1 3,381,642 - BPHYT_RS34650 0.81 +0.4 3,381,712 - BPHYT_RS34650 0.88 -0.5 3,381,853 + +0.4 3,381,854 - +0.9 3,382,236 + BPHYT_RS34655 0.32 -2.1 3,382,780 + BPHYT_RS34655 0.78 -1.6
Or see this region's nucleotide sequence