Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33615

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33610 and BPHYT_RS33615 are separated by 1 nucleotidesBPHYT_RS33615 and BPHYT_RS33620 are separated by 60 nucleotides BPHYT_RS33610: BPHYT_RS33610 - DNA polymerase I, at 3,125,085 to 3,127,826 _RS33610 BPHYT_RS33615: BPHYT_RS33615 - decarboxylase, at 3,127,828 to 3,128,571 _RS33615 BPHYT_RS33620: BPHYT_RS33620 - transcriptional regulator, at 3,128,632 to 3,129,549 _RS33620 Position (kb) 3127 3128 3129Strain fitness (log2 ratio) -1 0 1 2at 3127.891 kb on - strandat 3127.987 kb on + strand, within BPHYT_RS33615at 3127.987 kb on + strand, within BPHYT_RS33615at 3127.987 kb on + strand, within BPHYT_RS33615at 3127.988 kb on - strand, within BPHYT_RS33615at 3127.988 kb on - strand, within BPHYT_RS33615at 3128.564 kb on - strandat 3128.623 kb on - strandat 3128.813 kb on - strand, within BPHYT_RS33620at 3128.885 kb on + strand, within BPHYT_RS33620at 3129.344 kb on + strand, within BPHYT_RS33620at 3129.345 kb on - strand, within BPHYT_RS33620at 3129.345 kb on - strand, within BPHYT_RS33620at 3129.561 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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3,127,891 - -0.3
3,127,987 + BPHYT_RS33615 0.21 +0.6
3,127,987 + BPHYT_RS33615 0.21 +1.8
3,127,987 + BPHYT_RS33615 0.21 -0.5
3,127,988 - BPHYT_RS33615 0.22 +2.7
3,127,988 - BPHYT_RS33615 0.22 +0.7
3,128,564 - +1.9
3,128,623 - -0.5
3,128,813 - BPHYT_RS33620 0.20 -0.2
3,128,885 + BPHYT_RS33620 0.28 -1.3
3,129,344 + BPHYT_RS33620 0.78 +0.8
3,129,345 - BPHYT_RS33620 0.78 +0.3
3,129,345 - BPHYT_RS33620 0.78 -0.3
3,129,561 + +1.5

Or see this region's nucleotide sequence