Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32970

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32960 and BPHYT_RS32965 are separated by 92 nucleotidesBPHYT_RS32965 and BPHYT_RS32970 are separated by 271 nucleotidesBPHYT_RS32970 and BPHYT_RS32980 are separated by 785 nucleotides BPHYT_RS32960: BPHYT_RS32960 - short-chain dehydrogenase, at 2,974,087 to 2,974,812 _RS32960 BPHYT_RS32965: BPHYT_RS32965 - HxlR family transcriptional regulator, at 2,974,905 to 2,975,300 _RS32965 BPHYT_RS32970: BPHYT_RS32970 - glutathione S-transferase, at 2,975,572 to 2,976,204 _RS32970 BPHYT_RS32980: BPHYT_RS32980 - carboxylesterase, at 2,976,990 to 2,978,699 _RS32980 Position (kb) 2975 2976 2977Strain fitness (log2 ratio) -1 0 1 2at 2974.833 kb on + strandat 2974.834 kb on - strandat 2974.852 kb on - strandat 2974.852 kb on - strandat 2974.861 kb on - strandat 2974.886 kb on - strandat 2975.342 kb on + strandat 2975.364 kb on - strandat 2975.364 kb on - strandat 2975.364 kb on - strandat 2975.803 kb on + strand, within BPHYT_RS32970at 2975.814 kb on + strand, within BPHYT_RS32970at 2976.086 kb on + strand, within BPHYT_RS32970at 2976.331 kb on - strandat 2976.584 kb on + strandat 2976.720 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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2,974,833 + +0.1
2,974,834 - -0.2
2,974,852 - -0.2
2,974,852 - +0.4
2,974,861 - +0.9
2,974,886 - -0.3
2,975,342 + +0.0
2,975,364 - +0.3
2,975,364 - -0.4
2,975,364 - +2.7
2,975,803 + BPHYT_RS32970 0.36 +0.6
2,975,814 + BPHYT_RS32970 0.38 +0.1
2,976,086 + BPHYT_RS32970 0.81 -0.1
2,976,331 - -0.1
2,976,584 + -1.1
2,976,720 + -0.1

Or see this region's nucleotide sequence