Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32925 and BPHYT_RS32930 are separated by 27 nucleotides BPHYT_RS32930 and BPHYT_RS32935 are separated by 57 nucleotides BPHYT_RS32935 and BPHYT_RS32940 are separated by 86 nucleotides
BPHYT_RS32925: BPHYT_RS32925 - nitrilotriacetate monooxygenase component A, at 2,967,080 to 2,968,426
_RS32925
BPHYT_RS32930: BPHYT_RS32930 - NADPH:quinone reductase and related Zn-dependent oxidoreductase-like protein, at 2,968,454 to 2,968,900
_RS32930
BPHYT_RS32935: BPHYT_RS32935 - 3-ketoacyl-ACP reductase, at 2,968,958 to 2,969,704
_RS32935
BPHYT_RS32940: BPHYT_RS32940 - MFS transporter, at 2,969,791 to 2,970,540
_RS32940
Position (kb)
2968
2969
2970 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2968.300 kb on - strand at 2968.341 kb on - strand at 2968.430 kb on - strand at 2968.481 kb on + strand at 2968.751 kb on - strand, within BPHYT_RS32930 at 2968.850 kb on - strand, within BPHYT_RS32930 at 2968.918 kb on + strand at 2968.919 kb on - strand at 2968.967 kb on - strand at 2969.134 kb on + strand, within BPHYT_RS32935 at 2969.255 kb on - strand, within BPHYT_RS32935 at 2969.276 kb on - strand, within BPHYT_RS32935 at 2969.309 kb on + strand, within BPHYT_RS32935 at 2969.497 kb on + strand, within BPHYT_RS32935 at 2970.107 kb on - strand, within BPHYT_RS32940 at 2970.423 kb on + strand, within BPHYT_RS32940 at 2970.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,968,300 - +0.3 2,968,341 - -0.3 2,968,430 - -1.0 2,968,481 + -0.2 2,968,751 - BPHYT_RS32930 0.66 +0.3 2,968,850 - BPHYT_RS32930 0.89 -2.1 2,968,918 + +0.1 2,968,919 - +2.9 2,968,967 - +1.2 2,969,134 + BPHYT_RS32935 0.24 +0.5 2,969,255 - BPHYT_RS32935 0.40 +0.4 2,969,276 - BPHYT_RS32935 0.43 -1.5 2,969,309 + BPHYT_RS32935 0.47 -1.5 2,969,497 + BPHYT_RS32935 0.72 +0.1 2,970,107 - BPHYT_RS32940 0.42 -0.3 2,970,423 + BPHYT_RS32940 0.84 +0.1 2,970,492 + -1.0
Or see this region's nucleotide sequence