Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32145 and BPHYT_RS32150 overlap by 4 nucleotides BPHYT_RS32150 and BPHYT_RS32155 are separated by 7 nucleotides BPHYT_RS32155 and BPHYT_RS32160 overlap by 4 nucleotides
BPHYT_RS32145: BPHYT_RS32145 - type IV secretory pathway protein VirD4, at 2,785,143 to 2,786,894
_RS32145
BPHYT_RS32150: BPHYT_RS32150 - hypothetical protein, at 2,786,891 to 2,787,295
_RS32150
BPHYT_RS32155: BPHYT_RS32155 - type IV secretion system protein VirB11, at 2,787,303 to 2,788,421
_RS32155
BPHYT_RS32160: BPHYT_RS32160 - type IV secretion system protein VirB10, at 2,788,418 to 2,789,662
_RS32160
Position (kb)
2787
2788
2789 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2786.414 kb on - strand, within BPHYT_RS32145 at 2786.581 kb on - strand, within BPHYT_RS32145 at 2786.740 kb on + strand at 2786.847 kb on + strand at 2786.946 kb on + strand, within BPHYT_RS32150 at 2787.006 kb on - strand, within BPHYT_RS32150 at 2787.147 kb on + strand, within BPHYT_RS32150 at 2787.710 kb on - strand, within BPHYT_RS32155 at 2787.816 kb on - strand, within BPHYT_RS32155 at 2787.816 kb on - strand, within BPHYT_RS32155 at 2788.158 kb on + strand, within BPHYT_RS32155 at 2788.158 kb on + strand, within BPHYT_RS32155 at 2788.587 kb on + strand, within BPHYT_RS32160 at 2788.833 kb on + strand, within BPHYT_RS32160 at 2789.235 kb on + strand, within BPHYT_RS32160 at 2789.238 kb on + strand, within BPHYT_RS32160 at 2789.238 kb on + strand, within BPHYT_RS32160
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,786,414 - BPHYT_RS32145 0.73 -0.4 2,786,581 - BPHYT_RS32145 0.82 +0.1 2,786,740 + -1.4 2,786,847 + -2.3 2,786,946 + BPHYT_RS32150 0.14 +2.1 2,787,006 - BPHYT_RS32150 0.28 +0.5 2,787,147 + BPHYT_RS32150 0.63 -1.9 2,787,710 - BPHYT_RS32155 0.36 +1.0 2,787,816 - BPHYT_RS32155 0.46 -0.2 2,787,816 - BPHYT_RS32155 0.46 +0.3 2,788,158 + BPHYT_RS32155 0.76 -0.9 2,788,158 + BPHYT_RS32155 0.76 +0.5 2,788,587 + BPHYT_RS32160 0.14 -0.2 2,788,833 + BPHYT_RS32160 0.33 -0.1 2,789,235 + BPHYT_RS32160 0.66 -0.1 2,789,238 + BPHYT_RS32160 0.66 -1.2 2,789,238 + BPHYT_RS32160 0.66 -0.3
Or see this region's nucleotide sequence