Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31115 and BPHYT_RS31120 are separated by 120 nucleotides BPHYT_RS31120 and BPHYT_RS31125 are separated by 71 nucleotides BPHYT_RS31125 and BPHYT_RS31130 are separated by 100 nucleotides
BPHYT_RS31115: BPHYT_RS31115 - glucosidase, at 2,535,852 to 2,538,593
_RS31115
BPHYT_RS31120: BPHYT_RS31120 - pseudouridine-5'-phosphate glycosidase, at 2,538,714 to 2,539,667
_RS31120
BPHYT_RS31125: BPHYT_RS31125 - hypothetical protein, at 2,539,739 to 2,540,188
_RS31125
BPHYT_RS31130: BPHYT_RS31130 - hypothetical protein, at 2,540,289 to 2,540,492
_RS31130
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2537.898 kb on - strand, within BPHYT_RS31115 at 2538.033 kb on - strand, within BPHYT_RS31115 at 2538.033 kb on - strand, within BPHYT_RS31115 at 2538.187 kb on + strand, within BPHYT_RS31115 at 2538.193 kb on + strand, within BPHYT_RS31115 at 2538.209 kb on - strand, within BPHYT_RS31115 at 2538.452 kb on - strand at 2538.458 kb on + strand at 2538.623 kb on + strand at 2538.624 kb on - strand at 2539.060 kb on + strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.061 kb on - strand, within BPHYT_RS31120 at 2539.699 kb on - strand at 2539.700 kb on + strand at 2539.700 kb on + strand at 2539.786 kb on + strand, within BPHYT_RS31125 at 2539.787 kb on - strand, within BPHYT_RS31125 at 2539.888 kb on - strand, within BPHYT_RS31125 at 2540.212 kb on - strand at 2540.328 kb on + strand, within BPHYT_RS31130 at 2540.433 kb on - strand, within BPHYT_RS31130 at 2540.433 kb on - strand, within BPHYT_RS31130 at 2540.434 kb on + strand, within BPHYT_RS31130 at 2540.492 kb on + strand at 2540.492 kb on + strand at 2540.560 kb on - strand at 2540.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,537,898 - BPHYT_RS31115 0.75 +0.1 2,538,033 - BPHYT_RS31115 0.80 -0.8 2,538,033 - BPHYT_RS31115 0.80 -1.3 2,538,187 + BPHYT_RS31115 0.85 +2.2 2,538,193 + BPHYT_RS31115 0.85 +0.4 2,538,209 - BPHYT_RS31115 0.86 -1.5 2,538,452 - -0.5 2,538,458 + +0.4 2,538,623 + +0.1 2,538,624 - -0.0 2,539,060 + BPHYT_RS31120 0.36 -0.3 2,539,061 - BPHYT_RS31120 0.36 -1.3 2,539,061 - BPHYT_RS31120 0.36 -0.1 2,539,061 - BPHYT_RS31120 0.36 +0.1 2,539,699 - -1.4 2,539,700 + +0.1 2,539,700 + -1.2 2,539,786 + BPHYT_RS31125 0.10 -1.6 2,539,787 - BPHYT_RS31125 0.11 -1.0 2,539,888 - BPHYT_RS31125 0.33 -2.1 2,540,212 - +0.2 2,540,328 + BPHYT_RS31130 0.19 -0.3 2,540,433 - BPHYT_RS31130 0.71 -0.6 2,540,433 - BPHYT_RS31130 0.71 +1.5 2,540,434 + BPHYT_RS31130 0.71 +0.4 2,540,492 + +1.1 2,540,492 + -0.3 2,540,560 - -3.0 2,540,597 + -0.1
Or see this region's nucleotide sequence