Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30115 and BPHYT_RS30120 are separated by 169 nucleotides BPHYT_RS30120 and BPHYT_RS30125 are separated by 29 nucleotides BPHYT_RS30125 and BPHYT_RS30130 are separated by 94 nucleotides BPHYT_RS30130 and BPHYT_RS30135 are separated by 230 nucleotides
BPHYT_RS30115: BPHYT_RS30115 - hypothetical protein, at 2,310,137 to 2,310,559
_RS30115
BPHYT_RS30120: BPHYT_RS30120 - heavy metal transporter, at 2,310,729 to 2,310,926
_RS30120
BPHYT_RS30125: BPHYT_RS30125 - hypothetical protein, at 2,310,956 to 2,311,369
_RS30125
BPHYT_RS30130: BPHYT_RS30130 - AraC family transcriptional regulator, at 2,311,464 to 2,312,450
_RS30130
BPHYT_RS30135: BPHYT_RS30135 - SAM-dependent methyltransferase, at 2,312,681 to 2,313,514
_RS30135
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-1
0
1 at 2310.563 kb on + strand at 2310.572 kb on + strand at 2310.573 kb on - strand at 2310.749 kb on + strand, within BPHYT_RS30120 at 2310.852 kb on - strand, within BPHYT_RS30120 at 2311.656 kb on - strand, within BPHYT_RS30130 at 2311.845 kb on - strand, within BPHYT_RS30130 at 2312.093 kb on - strand, within BPHYT_RS30130 at 2312.765 kb on + strand, within BPHYT_RS30135 at 2313.109 kb on - strand, within BPHYT_RS30135 at 2313.356 kb on + strand, within BPHYT_RS30135
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,310,563 + -0.2 2,310,572 + -0.1 2,310,573 - -1.3 2,310,749 + BPHYT_RS30120 0.10 -0.2 2,310,852 - BPHYT_RS30120 0.62 -0.9 2,311,656 - BPHYT_RS30130 0.19 -0.6 2,311,845 - BPHYT_RS30130 0.39 -0.3 2,312,093 - BPHYT_RS30130 0.64 -1.6 2,312,765 + BPHYT_RS30135 0.10 -1.7 2,313,109 - BPHYT_RS30135 0.51 +0.8 2,313,356 + BPHYT_RS30135 0.81 -0.0
Or see this region's nucleotide sequence