Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27430

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27425 and BPHYT_RS27430 overlap by 4 nucleotidesBPHYT_RS27430 and BPHYT_RS27435 are separated by 294 nucleotides BPHYT_RS27425: BPHYT_RS27425 - C4-dicarboxylate ABC transporter, at 1,727,116 to 1,728,417 _RS27425 BPHYT_RS27430: BPHYT_RS27430 - LysR family transcriptional regulator, at 1,728,414 to 1,729,382 _RS27430 BPHYT_RS27435: BPHYT_RS27435 - amylo-alpha-1,6-glucosidase, at 1,729,677 to 1,731,950 _RS27435 Position (kb) 1728 1729 1730Strain fitness (log2 ratio) -2 -1 0 1at 1727.735 kb on + strand, within BPHYT_RS27425at 1728.069 kb on + strand, within BPHYT_RS27425at 1728.070 kb on - strand, within BPHYT_RS27425at 1728.247 kb on - strand, within BPHYT_RS27425at 1728.386 kb on + strandat 1728.387 kb on - strandat 1728.539 kb on + strand, within BPHYT_RS27430at 1729.583 kb on - strandat 1729.596 kb on + strandat 1729.597 kb on - strandat 1729.597 kb on - strandat 1729.597 kb on - strandat 1729.631 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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1,727,735 + BPHYT_RS27425 0.48 -0.5
1,728,069 + BPHYT_RS27425 0.73 +1.6
1,728,070 - BPHYT_RS27425 0.73 +0.8
1,728,247 - BPHYT_RS27425 0.87 -0.2
1,728,386 + -0.4
1,728,387 - +0.4
1,728,539 + BPHYT_RS27430 0.13 -2.1
1,729,583 - -1.4
1,729,596 + +0.4
1,729,597 - +0.9
1,729,597 - -0.1
1,729,597 - -2.2
1,729,631 + -0.4

Or see this region's nucleotide sequence