Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26385

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26380 and BPHYT_RS26385 are separated by 27 nucleotidesBPHYT_RS26385 and BPHYT_RS26390 are separated by 87 nucleotides BPHYT_RS26380: BPHYT_RS26380 - cobalamin biosynthesis protein CobT, at 1,491,243 to 1,492,991 _RS26380 BPHYT_RS26385: BPHYT_RS26385 - glutamine amidotransferase, at 1,493,019 to 1,493,852 _RS26385 BPHYT_RS26390: BPHYT_RS26390 - antibiotic resistance protein, at 1,493,940 to 1,494,617 _RS26390 Position (kb) 1493 1494Strain fitness (log2 ratio) -2 -1 0 1at 1492.629 kb on - strand, within BPHYT_RS26380at 1493.498 kb on - strand, within BPHYT_RS26385at 1493.853 kb on - strandat 1493.853 kb on - strandat 1494.182 kb on + strand, within BPHYT_RS26390at 1494.617 kb on + strandat 1494.777 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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1,492,629 - BPHYT_RS26380 0.79 -1.2
1,493,498 - BPHYT_RS26385 0.57 -1.8
1,493,853 - -0.0
1,493,853 - +0.8
1,494,182 + BPHYT_RS26390 0.36 +1.2
1,494,617 + +1.1
1,494,777 + +0.8

Or see this region's nucleotide sequence