Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24725 and BPHYT_RS24730 are separated by 207 nucleotides BPHYT_RS24730 and BPHYT_RS24735 are separated by 93 nucleotides BPHYT_RS24735 and BPHYT_RS24740 overlap by 4 nucleotides
BPHYT_RS24725: BPHYT_RS24725 - NmrA family transcriptional regulator, at 1,113,598 to 1,114,521
_RS24725
BPHYT_RS24730: BPHYT_RS24730 - AraC family transcriptional regulator, at 1,114,729 to 1,115,685
_RS24730
BPHYT_RS24735: BPHYT_RS24735 - transcriptional regulator, at 1,115,779 to 1,116,606
_RS24735
BPHYT_RS24740: BPHYT_RS24740 - RNA polymerase subunit sigma-24, at 1,116,603 to 1,117,121
_RS24740
Position (kb)
1114
1115
1116 Strain fitness (log2 ratio)
-1
0
1 at 1113.911 kb on - strand, within BPHYT_RS24725 at 1114.066 kb on + strand, within BPHYT_RS24725 at 1114.067 kb on - strand, within BPHYT_RS24725 at 1114.086 kb on + strand, within BPHYT_RS24725 at 1114.087 kb on - strand, within BPHYT_RS24725 at 1114.558 kb on + strand at 1114.624 kb on + strand at 1114.656 kb on - strand at 1114.659 kb on + strand at 1114.884 kb on + strand, within BPHYT_RS24730 at 1114.885 kb on - strand, within BPHYT_RS24730 at 1114.900 kb on + strand, within BPHYT_RS24730 at 1115.242 kb on - strand, within BPHYT_RS24730 at 1115.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,113,911 - BPHYT_RS24725 0.34 +0.2 1,114,066 + BPHYT_RS24725 0.51 +0.2 1,114,067 - BPHYT_RS24725 0.51 -0.9 1,114,086 + BPHYT_RS24725 0.53 +0.4 1,114,087 - BPHYT_RS24725 0.53 -0.4 1,114,558 + +0.9 1,114,624 + -1.5 1,114,656 - +1.6 1,114,659 + -0.8 1,114,884 + BPHYT_RS24730 0.16 -1.6 1,114,885 - BPHYT_RS24730 0.16 -0.9 1,114,900 + BPHYT_RS24730 0.18 -0.7 1,115,242 - BPHYT_RS24730 0.54 -0.6 1,115,634 + -0.2
Or see this region's nucleotide sequence