Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotides BPHYT_RS24175 and BPHYT_RS24180 are separated by 153 nucleotides BPHYT_RS24180 and BPHYT_RS24185 are separated by 7 nucleotides
BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737
_RS24170
BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317
_RS24175
BPHYT_RS24180: BPHYT_RS24180 - C4-dicarboxylate ABC transporter substrate-binding protein, at 993,471 to 993,950
_RS24180
BPHYT_RS24185: BPHYT_RS24185 - C4-dicarboxylate ABC transporter permease, at 993,958 to 995,310
_RS24185
Position (kb) 992
993
994 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 991.342 kb on + strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.994 kb on - strand at 992.040 kb on + strand at 992.330 kb on + strand at 992.331 kb on - strand at 992.446 kb on - strand, within BPHYT_RS24175 at 992.531 kb on + strand, within BPHYT_RS24175 at 992.784 kb on + strand, within BPHYT_RS24175 at 992.917 kb on - strand, within BPHYT_RS24175
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 991,342 + BPHYT_RS24170 0.57 -1.1 991,343 - BPHYT_RS24170 0.58 -2.4 991,343 - BPHYT_RS24170 0.58 -0.3 991,994 - -1.5 992,040 + +3.6 992,330 + -0.6 992,331 - +0.6 992,446 - BPHYT_RS24175 0.12 -0.9 992,531 + BPHYT_RS24175 0.21 +0.3 992,784 + BPHYT_RS24175 0.46 -0.2 992,917 - BPHYT_RS24175 0.60 -0.1
Or see this region's nucleotide sequence