Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20965 and BPHYT_RS20970 are separated by 70 nucleotides BPHYT_RS20970 and BPHYT_RS20975 are separated by 138 nucleotides BPHYT_RS20975 and BPHYT_RS20980 are separated by 692 nucleotides
BPHYT_RS20965: BPHYT_RS20965 - photosystem reaction center subunit H, at 263,954 to 264,427
_RS20965
BPHYT_RS20970: BPHYT_RS20970 - hypothetical protein, at 264,498 to 264,728
_RS20970
BPHYT_RS20975: BPHYT_RS20975 - haloacid dehalogenase, at 264,867 to 265,595
_RS20975
BPHYT_RS20980: BPHYT_RS20980 - hypothetical protein, at 266,288 to 266,614
_RS20980
Position (kb) 264
265
266 Strain fitness (log2 ratio) -1
0
1 at 263.943 kb on - strand at 263.946 kb on - strand at 264.031 kb on - strand, within BPHYT_RS20965 at 264.452 kb on - strand at 264.659 kb on + strand, within BPHYT_RS20970 at 264.855 kb on + strand at 265.096 kb on + strand, within BPHYT_RS20975 at 265.096 kb on + strand, within BPHYT_RS20975 at 265.417 kb on - strand, within BPHYT_RS20975 at 265.696 kb on + strand at 265.824 kb on + strand at 265.827 kb on + strand at 265.828 kb on - strand at 265.887 kb on + strand at 265.959 kb on + strand at 265.963 kb on + strand at 265.964 kb on - strand at 265.964 kb on - strand at 265.964 kb on - strand at 266.001 kb on + strand at 266.022 kb on + strand at 266.022 kb on + strand at 266.275 kb on - strand at 266.580 kb on + strand, within BPHYT_RS20980
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 263,943 - -1.0 263,946 - +0.0 264,031 - BPHYT_RS20965 0.16 -0.9 264,452 - -0.3 264,659 + BPHYT_RS20970 0.70 +1.1 264,855 + +0.1 265,096 + BPHYT_RS20975 0.31 +0.5 265,096 + BPHYT_RS20975 0.31 +0.1 265,417 - BPHYT_RS20975 0.75 +0.4 265,696 + +0.1 265,824 + +0.5 265,827 + -0.2 265,828 - +0.3 265,887 + +1.1 265,959 + -1.4 265,963 + +0.6 265,964 - +1.0 265,964 - -1.0 265,964 - -0.9 266,001 + -1.3 266,022 + -0.7 266,022 + -0.2 266,275 - +0.4 266,580 + BPHYT_RS20980 0.89 +0.6
Or see this region's nucleotide sequence