Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15870

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15865 and BPHYT_RS15870 are separated by 566 nucleotidesBPHYT_RS15870 and BPHYT_RS15875 overlap by 20 nucleotidesBPHYT_RS15875 and BPHYT_RS15880 are separated by 149 nucleotides BPHYT_RS15865: BPHYT_RS15865 - exodeoxyribonuclease VII large subunit, at 3,602,537 to 3,603,916 _RS15865 BPHYT_RS15870: BPHYT_RS15870 - tetraacyldisaccharide 4'-kinase, at 3,604,483 to 3,605,499 _RS15870 BPHYT_RS15875: BPHYT_RS15875 - hypothetical protein, at 3,605,480 to 3,605,683 _RS15875 BPHYT_RS15880: BPHYT_RS15880 - 3-deoxy-manno-octulosonate cytidylyltransferase 1, at 3,605,833 to 3,606,633 _RS15880 Position (kb) 3604 3605 3606Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3603.559 kb on - strand, within BPHYT_RS15865at 3603.624 kb on + strand, within BPHYT_RS15865at 3603.625 kb on - strand, within BPHYT_RS15865at 3603.748 kb on - strand, within BPHYT_RS15865at 3603.926 kb on - strandat 3603.926 kb on - strandat 3604.081 kb on + strandat 3604.189 kb on + strandat 3604.263 kb on - strandat 3605.462 kb on + strandat 3605.462 kb on + strandat 3605.462 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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3,603,559 - BPHYT_RS15865 0.74 +4.0
3,603,624 + BPHYT_RS15865 0.79 -0.7
3,603,625 - BPHYT_RS15865 0.79 -0.7
3,603,748 - BPHYT_RS15865 0.88 +0.4
3,603,926 - -0.0
3,603,926 - +0.2
3,604,081 + -1.9
3,604,189 + +0.2
3,604,263 - -1.8
3,605,462 + -0.4
3,605,462 + -1.1
3,605,462 + -0.4

Or see this region's nucleotide sequence