Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13835 and BPHYT_RS13840 overlap by 4 nucleotides BPHYT_RS13840 and BPHYT_RS13845 are separated by 274 nucleotides BPHYT_RS13845 and BPHYT_RS13850 overlap by 4 nucleotides
BPHYT_RS13835: BPHYT_RS13835 - S-adenosylhomocysteine nucleosidase, at 3,156,806 to 3,157,756
_RS13835
BPHYT_RS13840: BPHYT_RS13840 - DNA-dependent helicase, at 3,157,753 to 3,160,104
_RS13840
BPHYT_RS13845: BPHYT_RS13845 - transposase, at 3,160,379 to 3,160,681
_RS13845
BPHYT_RS13850: BPHYT_RS13850 - transposase, at 3,160,678 to 3,161,514
_RS13850
Position (kb) 3157
3158
3159
3160
3161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3156.771 kb on + strand at 3156.771 kb on + strand at 3156.771 kb on + strand at 3156.821 kb on - strand at 3156.821 kb on - strand at 3156.858 kb on + strand at 3156.859 kb on - strand at 3157.598 kb on + strand, within BPHYT_RS13835 at 3160.136 kb on + strand at 3160.136 kb on + strand at 3160.137 kb on - strand at 3160.137 kb on - strand at 3160.138 kb on + strand at 3160.139 kb on - strand at 3160.139 kb on - strand at 3160.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 3,156,771 + -0.4 3,156,771 + -1.7 3,156,771 + -1.0 3,156,821 - +1.8 3,156,821 - -0.2 3,156,858 + +0.2 3,156,859 - -3.0 3,157,598 + BPHYT_RS13835 0.83 -0.9 3,160,136 + -0.2 3,160,136 + +0.6 3,160,137 - +0.5 3,160,137 - +0.3 3,160,138 + -1.1 3,160,139 - +0.1 3,160,139 - +1.6 3,160,346 - -0.1
Or see this region's nucleotide sequence