Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12915 and BPHYT_RS12920 are separated by 89 nucleotides BPHYT_RS12920 and BPHYT_RS12925 are separated by 170 nucleotides BPHYT_RS12925 and BPHYT_RS12930 are separated by 21 nucleotides
BPHYT_RS12915: BPHYT_RS12915 - tRNA-Met, at 2,948,681 to 2,948,757
_RS12915
BPHYT_RS12920: BPHYT_RS12920 - hypothetical protein, at 2,948,847 to 2,949,134
_RS12920
BPHYT_RS12925: BPHYT_RS12925 - exodeoxyribonuclease III, at 2,949,305 to 2,950,081
_RS12925
BPHYT_RS12930: BPHYT_RS12930 - peptidase S9, at 2,950,103 to 2,952,202
_RS12930
Position (kb)
2949
2950
2951 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2948.390 kb on + strand at 2948.462 kb on + strand at 2948.651 kb on - strand at 2948.666 kb on - strand at 2948.776 kb on - strand at 2948.777 kb on + strand at 2948.902 kb on + strand, within BPHYT_RS12920 at 2949.182 kb on - strand at 2949.489 kb on - strand, within BPHYT_RS12925 at 2950.123 kb on + strand at 2950.347 kb on + strand, within BPHYT_RS12930 at 2950.348 kb on - strand, within BPHYT_RS12930 at 2950.409 kb on + strand, within BPHYT_RS12930 at 2950.472 kb on + strand, within BPHYT_RS12930 at 2950.472 kb on + strand, within BPHYT_RS12930 at 2950.473 kb on - strand, within BPHYT_RS12930 at 2950.473 kb on - strand, within BPHYT_RS12930 at 2950.702 kb on - strand, within BPHYT_RS12930 at 2950.746 kb on + strand, within BPHYT_RS12930 at 2950.747 kb on - strand, within BPHYT_RS12930
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,948,390 + -0.2 2,948,462 + -0.0 2,948,651 - -3.0 2,948,666 - -1.2 2,948,776 - +0.0 2,948,777 + +1.6 2,948,902 + BPHYT_RS12920 0.19 -0.0 2,949,182 - +0.2 2,949,489 - BPHYT_RS12925 0.24 -1.5 2,950,123 + -0.9 2,950,347 + BPHYT_RS12930 0.12 -1.8 2,950,348 - BPHYT_RS12930 0.12 -0.4 2,950,409 + BPHYT_RS12930 0.15 -0.5 2,950,472 + BPHYT_RS12930 0.18 -0.2 2,950,472 + BPHYT_RS12930 0.18 -1.1 2,950,473 - BPHYT_RS12930 0.18 +1.6 2,950,473 - BPHYT_RS12930 0.18 +0.5 2,950,702 - BPHYT_RS12930 0.29 +0.4 2,950,746 + BPHYT_RS12930 0.31 -3.0 2,950,747 - BPHYT_RS12930 0.31 -0.4
Or see this region's nucleotide sequence