Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12325 and BPHYT_RS12330 are separated by 142 nucleotides BPHYT_RS12330 and BPHYT_RS12335 are separated by 94 nucleotides BPHYT_RS12335 and BPHYT_RS12340 are separated by 61 nucleotides BPHYT_RS12340 and BPHYT_RS12345 are separated by 97 nucleotides BPHYT_RS12345 and BPHYT_RS12350 are separated by 155 nucleotides BPHYT_RS12350 and BPHYT_RS12355 are separated by 122 nucleotides
BPHYT_RS12325: BPHYT_RS12325 - GNAT family acetyltransferase, at 2,814,299 to 2,814,763
_RS12325
BPHYT_RS12330: BPHYT_RS12330 - tRNA-Asp, at 2,814,906 to 2,814,982
_RS12330
BPHYT_RS12335: BPHYT_RS12335 - tRNA-Glu, at 2,815,077 to 2,815,152
_RS12335
BPHYT_RS12340: BPHYT_RS12340 - tRNA-Asp, at 2,815,214 to 2,815,290
_RS12340
BPHYT_RS12345: BPHYT_RS12345 - tRNA-Glu, at 2,815,388 to 2,815,463
_RS12345
BPHYT_RS12350: BPHYT_RS12350 - tRNA-Ala, at 2,815,619 to 2,815,694
_RS12350
BPHYT_RS12355: BPHYT_RS12355 - glutamate--tRNA ligase, at 2,815,817 to 2,817,226
_RS12355
Position (kb)
2815
2816 Strain fitness (log2 ratio)
-2
-1
0
1 at 2814.657 kb on + strand, within BPHYT_RS12325 at 2814.657 kb on + strand, within BPHYT_RS12325 at 2814.840 kb on + strand at 2814.865 kb on + strand at 2815.088 kb on - strand, within BPHYT_RS12335 at 2815.165 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,814,657 + BPHYT_RS12325 0.77 +0.1 2,814,657 + BPHYT_RS12325 0.77 -2.3 2,814,840 + -0.3 2,814,865 + +1.6 2,815,088 - BPHYT_RS12335 0.14 -0.3 2,815,165 - -0.2
Or see this region's nucleotide sequence