Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12275 and BPHYT_RS12280 are separated by 527 nucleotides BPHYT_RS12280 and BPHYT_RS12285 are separated by 458 nucleotides
BPHYT_RS12275: BPHYT_RS12275 - acyl-CoA-binding protein, at 2,803,270 to 2,803,539
_RS12275
BPHYT_RS12280: BPHYT_RS12280 - RNA helicase, at 2,804,067 to 2,805,695
_RS12280
BPHYT_RS12285: BPHYT_RS12285 - isocitrate lyase, at 2,806,154 to 2,807,458
_RS12285
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2803.086 kb on - strand at 2803.218 kb on + strand at 2803.218 kb on + strand at 2803.435 kb on + strand, within BPHYT_RS12275 at 2803.435 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.441 kb on + strand, within BPHYT_RS12275 at 2803.495 kb on + strand, within BPHYT_RS12275 at 2803.705 kb on - strand at 2803.707 kb on + strand at 2803.733 kb on + strand at 2803.734 kb on - strand at 2803.734 kb on - strand at 2804.007 kb on + strand at 2804.042 kb on + strand at 2804.042 kb on + strand at 2804.053 kb on + strand at 2804.607 kb on + strand, within BPHYT_RS12280 at 2805.693 kb on + strand at 2805.693 kb on + strand at 2805.693 kb on + strand at 2805.697 kb on + strand at 2805.698 kb on - strand at 2805.698 kb on - strand at 2805.698 kb on - strand at 2805.711 kb on + strand at 2806.131 kb on + strand at 2806.232 kb on + strand at 2806.233 kb on - strand at 2806.233 kb on - strand at 2806.233 kb on - strand at 2806.409 kb on + strand, within BPHYT_RS12285 at 2806.583 kb on + strand, within BPHYT_RS12285 at 2806.584 kb on - strand, within BPHYT_RS12285 at 2806.593 kb on - strand, within BPHYT_RS12285 at 2806.593 kb on - strand, within BPHYT_RS12285
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,803,086 - +0.1 2,803,218 + -2.8 2,803,218 + -1.8 2,803,435 + BPHYT_RS12275 0.61 -0.5 2,803,435 + BPHYT_RS12275 0.61 +0.2 2,803,441 + BPHYT_RS12275 0.63 +0.2 2,803,441 + BPHYT_RS12275 0.63 +0.4 2,803,441 + BPHYT_RS12275 0.63 -2.9 2,803,495 + BPHYT_RS12275 0.83 -0.7 2,803,705 - -2.4 2,803,707 + -0.4 2,803,733 + -0.1 2,803,734 - -2.0 2,803,734 - -0.6 2,804,007 + -0.5 2,804,042 + -0.6 2,804,042 + +3.6 2,804,053 + -0.2 2,804,607 + BPHYT_RS12280 0.33 -2.2 2,805,693 + -1.1 2,805,693 + -0.8 2,805,693 + -1.2 2,805,697 + +0.2 2,805,698 - -0.4 2,805,698 - -0.5 2,805,698 - -0.0 2,805,711 + -0.0 2,806,131 + +0.6 2,806,232 + -0.9 2,806,233 - -0.4 2,806,233 - -0.8 2,806,233 - -1.0 2,806,409 + BPHYT_RS12285 0.20 +0.1 2,806,583 + BPHYT_RS12285 0.33 -0.2 2,806,584 - BPHYT_RS12285 0.33 +0.6 2,806,593 - BPHYT_RS12285 0.34 -0.5 2,806,593 - BPHYT_RS12285 0.34 +1.6
Or see this region's nucleotide sequence