Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12060 and BPHYT_RS12065 are separated by 160 nucleotides BPHYT_RS12065 and BPHYT_RS12070 are separated by 73 nucleotides BPHYT_RS12070 and BPHYT_RS12075 overlap by 4 nucleotides
BPHYT_RS12060: BPHYT_RS12060 - hypothetical protein, at 2,749,721 to 2,750,536
_RS12060
BPHYT_RS12065: BPHYT_RS12065 - RNA methyltransferase, at 2,750,697 to 2,751,482
_RS12065
BPHYT_RS12070: BPHYT_RS12070 - ribonuclease HII, at 2,751,556 to 2,752,302
_RS12070
BPHYT_RS12075: BPHYT_RS12075 - lipid-A-disaccharide synthase, at 2,752,299 to 2,753,468
_RS12075
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2749.734 kb on + strand at 2750.078 kb on + strand, within BPHYT_RS12060 at 2750.078 kb on + strand, within BPHYT_RS12060 at 2750.521 kb on + strand at 2750.522 kb on - strand at 2750.531 kb on + strand at 2751.120 kb on + strand, within BPHYT_RS12065 at 2751.120 kb on + strand, within BPHYT_RS12065 at 2751.121 kb on - strand, within BPHYT_RS12065 at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.448 kb on + strand at 2751.449 kb on - strand at 2751.535 kb on + strand at 2751.536 kb on - strand at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.100 kb on - strand, within BPHYT_RS12070 at 2752.276 kb on - strand at 2752.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 2,749,734 + +0.2 2,750,078 + BPHYT_RS12060 0.44 -0.9 2,750,078 + BPHYT_RS12060 0.44 +4.1 2,750,521 + -0.7 2,750,522 - +1.1 2,750,531 + -1.1 2,751,120 + BPHYT_RS12065 0.54 -1.6 2,751,120 + BPHYT_RS12065 0.54 +2.5 2,751,121 - BPHYT_RS12065 0.54 -1.5 2,751,404 + -0.2 2,751,404 + +0.3 2,751,404 + +0.2 2,751,404 + -1.7 2,751,448 + +0.2 2,751,449 - -0.8 2,751,535 + +0.1 2,751,536 - -0.1 2,752,040 + BPHYT_RS12070 0.65 -0.2 2,752,040 + BPHYT_RS12070 0.65 -0.5 2,752,100 - BPHYT_RS12070 0.73 +0.5 2,752,276 - +0.4 2,752,276 - +1.0
Or see this region's nucleotide sequence