Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10065

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10060 and BPHYT_RS10065 are separated by 1 nucleotidesBPHYT_RS10065 and BPHYT_RS10070 are separated by 44 nucleotidesBPHYT_RS10070 and BPHYT_RS10075 are separated by 98 nucleotides BPHYT_RS10060: BPHYT_RS10060 - cytochrome C oxidase, at 2,290,675 to 2,292,660 _RS10060 BPHYT_RS10065: BPHYT_RS10065 - cytochrome C oxidase subunit I, at 2,292,662 to 2,293,306 _RS10065 BPHYT_RS10070: BPHYT_RS10070 - hypothetical protein, at 2,293,351 to 2,293,734 _RS10070 BPHYT_RS10075: BPHYT_RS10075 - alcohol dehydrogenase, at 2,293,833 to 2,295,134 _RS10075 Position (kb) 2292 2293 2294Strain fitness (log2 ratio) -1 0 1 2at 2292.093 kb on + strand, within BPHYT_RS10060at 2292.093 kb on + strand, within BPHYT_RS10060at 2293.005 kb on + strand, within BPHYT_RS10065at 2293.080 kb on - strand, within BPHYT_RS10065at 2293.102 kb on + strand, within BPHYT_RS10065at 2293.514 kb on + strand, within BPHYT_RS10070at 2293.515 kb on - strand, within BPHYT_RS10070at 2293.749 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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2,292,093 + BPHYT_RS10060 0.71 -0.1
2,292,093 + BPHYT_RS10060 0.71 -1.4
2,293,005 + BPHYT_RS10065 0.53 +0.4
2,293,080 - BPHYT_RS10065 0.65 +0.4
2,293,102 + BPHYT_RS10065 0.68 +0.1
2,293,514 + BPHYT_RS10070 0.42 +2.7
2,293,515 - BPHYT_RS10070 0.43 +0.1
2,293,749 - -1.1

Or see this region's nucleotide sequence