Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09505

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09500 and BPHYT_RS09505 are separated by 234 nucleotidesBPHYT_RS09505 and BPHYT_RS09510 are separated by 119 nucleotides BPHYT_RS09500: BPHYT_RS09500 - glucose-6-phosphate isomerase, at 2,151,729 to 2,153,351 _RS09500 BPHYT_RS09505: BPHYT_RS09505 - carbohydrate kinase, at 2,153,586 to 2,155,175 _RS09505 BPHYT_RS09510: BPHYT_RS09510 - amino acid dehydrogenase, at 2,155,295 to 2,156,593 _RS09510 Position (kb) 2153 2154 2155 2156Strain fitness (log2 ratio) -1 0 1 2 3at 2152.945 kb on + strand, within BPHYT_RS09500at 2153.020 kb on + strand, within BPHYT_RS09500at 2153.305 kb on + strandat 2153.306 kb on - strandat 2153.352 kb on - strandat 2153.427 kb on + strandat 2153.427 kb on + strandat 2153.428 kb on - strandat 2153.428 kb on - strandat 2153.519 kb on + strandat 2153.519 kb on + strandat 2153.519 kb on + strandat 2153.717 kb on - strandat 2153.886 kb on + strand, within BPHYT_RS09505at 2153.886 kb on + strand, within BPHYT_RS09505at 2153.887 kb on - strand, within BPHYT_RS09505at 2154.021 kb on + strand, within BPHYT_RS09505at 2154.021 kb on + strand, within BPHYT_RS09505at 2154.021 kb on + strand, within BPHYT_RS09505at 2154.021 kb on + strand, within BPHYT_RS09505at 2154.021 kb on + strand, within BPHYT_RS09505at 2154.022 kb on - strand, within BPHYT_RS09505at 2154.244 kb on + strand, within BPHYT_RS09505at 2154.557 kb on + strand, within BPHYT_RS09505at 2154.989 kb on + strand, within BPHYT_RS09505at 2154.999 kb on - strand, within BPHYT_RS09505at 2155.228 kb on + strandat 2155.229 kb on - strandat 2155.233 kb on + strandat 2155.297 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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2,152,945 + BPHYT_RS09500 0.75 +2.6
2,153,020 + BPHYT_RS09500 0.80 +1.7
2,153,305 + +2.5
2,153,306 - +1.9
2,153,352 - +3.1
2,153,427 + +1.9
2,153,427 + +2.1
2,153,428 - -0.2
2,153,428 - -0.3
2,153,519 + -0.1
2,153,519 + -1.1
2,153,519 + -1.0
2,153,717 - -1.7
2,153,886 + BPHYT_RS09505 0.19 +2.5
2,153,886 + BPHYT_RS09505 0.19 +0.3
2,153,887 - BPHYT_RS09505 0.19 -1.1
2,154,021 + BPHYT_RS09505 0.27 +0.3
2,154,021 + BPHYT_RS09505 0.27 +0.2
2,154,021 + BPHYT_RS09505 0.27 -0.5
2,154,021 + BPHYT_RS09505 0.27 -0.0
2,154,021 + BPHYT_RS09505 0.27 +0.2
2,154,022 - BPHYT_RS09505 0.27 +0.4
2,154,244 + BPHYT_RS09505 0.41 -0.9
2,154,557 + BPHYT_RS09505 0.61 +2.7
2,154,989 + BPHYT_RS09505 0.88 -0.6
2,154,999 - BPHYT_RS09505 0.89 +1.3
2,155,228 + +0.1
2,155,229 - -0.5
2,155,233 + -0.2
2,155,297 - +0.8

Or see this region's nucleotide sequence