Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08645

Experiment: 36hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08640 and BPHYT_RS08645 are separated by 51 nucleotidesBPHYT_RS08645 and BPHYT_RS08650 overlap by 4 nucleotides BPHYT_RS08640: BPHYT_RS08640 - alkanesulfonate monooxygenase, at 1,969,223 to 1,970,383 _RS08640 BPHYT_RS08645: BPHYT_RS08645 - sulfonate ABC transporter, at 1,970,435 to 1,971,277 _RS08645 BPHYT_RS08650: BPHYT_RS08650 - sulfonate ABC transporter ATP-binding protein, at 1,971,274 to 1,972,275 _RS08650 Position (kb) 1970 1971 1972Strain fitness (log2 ratio) -3 -2 -1 0 1at 1969.719 kb on - strand, within BPHYT_RS08640at 1969.798 kb on + strand, within BPHYT_RS08640at 1969.798 kb on + strand, within BPHYT_RS08640at 1970.253 kb on - strand, within BPHYT_RS08640at 1970.354 kb on + strandat 1970.355 kb on - strandat 1970.355 kb on - strandat 1970.906 kb on + strand, within BPHYT_RS08645at 1971.255 kb on - strandat 1971.392 kb on - strand, within BPHYT_RS08650

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1
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1,969,719 - BPHYT_RS08640 0.43 -2.8
1,969,798 + BPHYT_RS08640 0.50 +0.2
1,969,798 + BPHYT_RS08640 0.50 +0.4
1,970,253 - BPHYT_RS08640 0.89 +0.1
1,970,354 + -0.4
1,970,355 - -0.2
1,970,355 - -0.1
1,970,906 + BPHYT_RS08645 0.56 -2.2
1,971,255 - -0.3
1,971,392 - BPHYT_RS08650 0.12 +1.0

Or see this region's nucleotide sequence