Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08465 and BPHYT_RS08470 are separated by 472 nucleotides BPHYT_RS08470 and BPHYT_RS08475 are separated by 197 nucleotides BPHYT_RS08475 and BPHYT_RS08480 overlap by 4 nucleotides
BPHYT_RS08465: BPHYT_RS08465 - ribose-5-phosphate isomerase A, at 1,936,465 to 1,937,160
_RS08465
BPHYT_RS08470: BPHYT_RS08470 - glycosyl transferase, at 1,937,633 to 1,938,829
_RS08470
BPHYT_RS08475: BPHYT_RS08475 - hypothetical protein, at 1,939,027 to 1,939,326
_RS08475
BPHYT_RS08480: BPHYT_RS08480 - transcriptional regulator, at 1,939,323 to 1,939,745
_RS08480
Position (kb) 1937
1938
1939 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1937.271 kb on - strand at 1937.284 kb on - strand at 1937.284 kb on - strand at 1937.584 kb on + strand at 1937.584 kb on + strand at 1937.584 kb on + strand at 1937.585 kb on - strand at 1937.759 kb on - strand, within BPHYT_RS08470 at 1937.834 kb on + strand, within BPHYT_RS08470 at 1937.883 kb on - strand, within BPHYT_RS08470 at 1937.894 kb on - strand, within BPHYT_RS08470 at 1937.897 kb on - strand, within BPHYT_RS08470 at 1937.950 kb on + strand, within BPHYT_RS08470 at 1938.899 kb on + strand at 1938.907 kb on + strand at 1939.139 kb on - strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.189 kb on + strand, within BPHYT_RS08475 at 1939.243 kb on + strand, within BPHYT_RS08475 at 1939.244 kb on - strand, within BPHYT_RS08475 at 1939.275 kb on - strand, within BPHYT_RS08475 at 1939.291 kb on + strand, within BPHYT_RS08475 at 1939.426 kb on - strand, within BPHYT_RS08480 at 1939.627 kb on + strand, within BPHYT_RS08480
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,937,271 - +0.6 1,937,284 - -1.6 1,937,284 - -2.6 1,937,584 + +0.0 1,937,584 + -2.2 1,937,584 + +0.1 1,937,585 - +0.3 1,937,759 - BPHYT_RS08470 0.11 +0.5 1,937,834 + BPHYT_RS08470 0.17 +2.0 1,937,883 - BPHYT_RS08470 0.21 -0.2 1,937,894 - BPHYT_RS08470 0.22 +1.4 1,937,897 - BPHYT_RS08470 0.22 -0.9 1,937,950 + BPHYT_RS08470 0.26 +0.7 1,938,899 + +0.1 1,938,907 + -1.9 1,939,139 - BPHYT_RS08475 0.37 -0.4 1,939,174 + BPHYT_RS08475 0.49 +0.0 1,939,174 + BPHYT_RS08475 0.49 +0.4 1,939,189 + BPHYT_RS08475 0.54 +1.6 1,939,243 + BPHYT_RS08475 0.72 +2.4 1,939,244 - BPHYT_RS08475 0.72 -0.3 1,939,275 - BPHYT_RS08475 0.83 +1.3 1,939,291 + BPHYT_RS08475 0.88 -0.8 1,939,426 - BPHYT_RS08480 0.24 +4.1 1,939,627 + BPHYT_RS08480 0.72 -0.5
Or see this region's nucleotide sequence