Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05680 and BPHYT_RS05685 are separated by 52 nucleotides BPHYT_RS05685 and BPHYT_RS05690 are separated by 20 nucleotides BPHYT_RS05690 and BPHYT_RS05695 are separated by 548 nucleotides
BPHYT_RS05680: BPHYT_RS05680 - DNA-cytosine methyltransferase, at 1,297,067 to 1,298,020
_RS05680
BPHYT_RS05685: BPHYT_RS05685 - hypothetical protein, at 1,298,073 to 1,298,306
_RS05685
BPHYT_RS05690: BPHYT_RS05690 - hypothetical protein, at 1,298,327 to 1,298,698
_RS05690
BPHYT_RS05695: BPHYT_RS05695 - transcriptional regulator, at 1,299,247 to 1,300,137
_RS05695
Position (kb)
1298
1299 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1298.043 kb on - strand at 1298.043 kb on - strand at 1298.044 kb on + strand at 1298.044 kb on + strand at 1298.044 kb on + strand at 1298.045 kb on - strand at 1298.045 kb on - strand at 1298.045 kb on - strand at 1298.047 kb on - strand at 1298.047 kb on - strand at 1298.047 kb on - strand at 1298.047 kb on - strand at 1298.284 kb on + strand at 1298.284 kb on + strand at 1298.285 kb on - strand at 1298.285 kb on - strand at 1298.294 kb on + strand at 1298.298 kb on + strand at 1298.298 kb on + strand at 1298.299 kb on - strand at 1298.299 kb on - strand at 1298.424 kb on + strand, within BPHYT_RS05690 at 1298.743 kb on + strand at 1298.941 kb on - strand at 1299.029 kb on + strand at 1299.177 kb on + strand at 1299.178 kb on - strand at 1299.259 kb on + strand at 1299.361 kb on + strand, within BPHYT_RS05695
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,298,043 - +1.0 1,298,043 - -1.5 1,298,044 + -0.6 1,298,044 + -2.4 1,298,044 + +0.1 1,298,045 - +0.4 1,298,045 - -0.2 1,298,045 - +0.1 1,298,047 - +1.0 1,298,047 - -1.5 1,298,047 - -0.2 1,298,047 - -0.8 1,298,284 + -0.9 1,298,284 + +0.5 1,298,285 - +1.2 1,298,285 - +0.0 1,298,294 + -0.9 1,298,298 + +0.6 1,298,298 + +2.0 1,298,299 - +0.6 1,298,299 - +0.4 1,298,424 + BPHYT_RS05690 0.26 -3.1 1,298,743 + +0.6 1,298,941 - -1.6 1,299,029 + +0.3 1,299,177 + -0.2 1,299,178 - -0.1 1,299,259 + +1.6 1,299,361 + BPHYT_RS05695 0.13 -0.5
Or see this region's nucleotide sequence