Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05515 and BPHYT_RS05520 are separated by 220 nucleotides BPHYT_RS05520 and BPHYT_RS05525 overlap by 17 nucleotides BPHYT_RS05525 and BPHYT_RS05530 are separated by 91 nucleotides BPHYT_RS05530 and BPHYT_RS05535 are separated by 211 nucleotides
BPHYT_RS05515: BPHYT_RS05515 - hypothetical protein, at 1,266,556 to 1,267,086
_RS05515
BPHYT_RS05520: BPHYT_RS05520 - transcriptional regulator, at 1,267,307 to 1,268,032
_RS05520
BPHYT_RS05525: BPHYT_RS05525 - sensor histidine kinase, at 1,268,016 to 1,269,335
_RS05525
BPHYT_RS05530: BPHYT_RS05530 - hypothetical protein, at 1,269,427 to 1,269,693
_RS05530
BPHYT_RS05535: BPHYT_RS05535 - diguanylate cyclase, at 1,269,905 to 1,271,596
_RS05535
Position (kb)
1268
1269
1270 Strain fitness (log2 ratio)
-2
-1
0
1 at 1267.080 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.189 kb on - strand at 1267.207 kb on - strand at 1267.220 kb on - strand at 1267.220 kb on - strand at 1267.313 kb on - strand at 1267.320 kb on + strand at 1267.320 kb on + strand at 1267.320 kb on + strand at 1267.321 kb on - strand at 1267.605 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1268.891 kb on - strand, within BPHYT_RS05525 at 1268.919 kb on - strand, within BPHYT_RS05525 at 1269.176 kb on + strand, within BPHYT_RS05525 at 1269.336 kb on + strand at 1269.336 kb on + strand at 1269.336 kb on + strand at 1269.337 kb on - strand at 1269.337 kb on - strand at 1269.429 kb on - strand at 1269.444 kb on + strand at 1269.444 kb on + strand at 1269.445 kb on - strand at 1269.478 kb on - strand, within BPHYT_RS05530 at 1269.509 kb on + strand, within BPHYT_RS05530 at 1269.521 kb on + strand, within BPHYT_RS05530 at 1269.522 kb on - strand, within BPHYT_RS05530 at 1269.624 kb on - strand, within BPHYT_RS05530 at 1269.731 kb on - strand at 1269.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,267,080 + -0.9 1,267,163 + -0.4 1,267,163 + -0.1 1,267,163 + +1.5 1,267,163 + -0.8 1,267,189 - -0.0 1,267,207 - -1.2 1,267,220 - +0.6 1,267,220 - +0.0 1,267,313 - +0.1 1,267,320 + -0.4 1,267,320 + -1.0 1,267,320 + -0.6 1,267,321 - +0.7 1,267,605 - BPHYT_RS05520 0.41 -0.4 1,267,881 - BPHYT_RS05520 0.79 +0.3 1,267,881 - BPHYT_RS05520 0.79 +0.6 1,267,881 - BPHYT_RS05520 0.79 -1.4 1,268,891 - BPHYT_RS05525 0.66 -2.2 1,268,919 - BPHYT_RS05525 0.68 -0.2 1,269,176 + BPHYT_RS05525 0.88 -0.9 1,269,336 + +1.4 1,269,336 + -1.0 1,269,336 + -0.7 1,269,337 - -1.8 1,269,337 - +0.4 1,269,429 - -0.1 1,269,444 + -0.8 1,269,444 + +0.2 1,269,445 - +0.8 1,269,478 - BPHYT_RS05530 0.19 +0.2 1,269,509 + BPHYT_RS05530 0.31 +1.0 1,269,521 + BPHYT_RS05530 0.35 +1.1 1,269,522 - BPHYT_RS05530 0.36 +0.6 1,269,624 - BPHYT_RS05530 0.74 +0.5 1,269,731 - -0.6 1,269,997 - -0.0
Or see this region's nucleotide sequence