Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05510 and BPHYT_RS05515 are separated by 200 nucleotides BPHYT_RS05515 and BPHYT_RS05520 are separated by 220 nucleotides BPHYT_RS05520 and BPHYT_RS05525 overlap by 17 nucleotides
BPHYT_RS05510: BPHYT_RS05510 - quercetin 2,3-dioxygenase, at 1,265,645 to 1,266,355
_RS05510
BPHYT_RS05515: BPHYT_RS05515 - hypothetical protein, at 1,266,556 to 1,267,086
_RS05515
BPHYT_RS05520: BPHYT_RS05520 - transcriptional regulator, at 1,267,307 to 1,268,032
_RS05520
BPHYT_RS05525: BPHYT_RS05525 - sensor histidine kinase, at 1,268,016 to 1,269,335
_RS05525
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-2
-1
0
1 at 1266.354 kb on - strand at 1266.391 kb on + strand at 1266.392 kb on - strand at 1266.460 kb on - strand at 1266.544 kb on - strand at 1266.701 kb on + strand, within BPHYT_RS05515 at 1266.701 kb on + strand, within BPHYT_RS05515 at 1266.701 kb on + strand, within BPHYT_RS05515 at 1266.702 kb on - strand, within BPHYT_RS05515 at 1266.702 kb on - strand, within BPHYT_RS05515 at 1267.080 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.163 kb on + strand at 1267.189 kb on - strand at 1267.207 kb on - strand at 1267.220 kb on - strand at 1267.220 kb on - strand at 1267.313 kb on - strand at 1267.320 kb on + strand at 1267.320 kb on + strand at 1267.320 kb on + strand at 1267.321 kb on - strand at 1267.605 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1267.881 kb on - strand, within BPHYT_RS05520 at 1268.891 kb on - strand, within BPHYT_RS05525 at 1268.919 kb on - strand, within BPHYT_RS05525
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,266,354 - +0.7 1,266,391 + +0.7 1,266,392 - -1.4 1,266,460 - -1.8 1,266,544 - +0.7 1,266,701 + BPHYT_RS05515 0.27 +0.1 1,266,701 + BPHYT_RS05515 0.27 +0.6 1,266,701 + BPHYT_RS05515 0.27 +0.2 1,266,702 - BPHYT_RS05515 0.27 +0.5 1,266,702 - BPHYT_RS05515 0.27 +1.4 1,267,080 + -0.9 1,267,163 + -0.4 1,267,163 + -0.1 1,267,163 + +1.5 1,267,163 + -0.8 1,267,189 - -0.0 1,267,207 - -1.2 1,267,220 - +0.6 1,267,220 - +0.0 1,267,313 - +0.1 1,267,320 + -0.4 1,267,320 + -1.0 1,267,320 + -0.6 1,267,321 - +0.7 1,267,605 - BPHYT_RS05520 0.41 -0.4 1,267,881 - BPHYT_RS05520 0.79 +0.3 1,267,881 - BPHYT_RS05520 0.79 +0.6 1,267,881 - BPHYT_RS05520 0.79 -1.4 1,268,891 - BPHYT_RS05525 0.66 -2.2 1,268,919 - BPHYT_RS05525 0.68 -0.2
Or see this region's nucleotide sequence