Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04330 and BPHYT_RS04335 are separated by 53 nucleotides BPHYT_RS04335 and BPHYT_RS04340 overlap by 4 nucleotides BPHYT_RS04340 and BPHYT_RS04345 are separated by 13 nucleotides
BPHYT_RS04330: BPHYT_RS04330 - hypothetical protein, at 997,214 to 998,356
_RS04330
BPHYT_RS04335: BPHYT_RS04335 - membrane protein, at 998,410 to 998,835
_RS04335
BPHYT_RS04340: BPHYT_RS04340 - hypothetical protein, at 998,832 to 1,000,115
_RS04340
BPHYT_RS04345: BPHYT_RS04345 - NAD-dependent epimerase/dehydratase, at 1,000,129 to 1,001,067
_RS04345
Position (kb)
998
999
1000
1001 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 998.117 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.204 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.529 kb on - strand, within BPHYT_RS04335 at 998.836 kb on - strand at 998.836 kb on - strand at 998.899 kb on + strand at 998.900 kb on - strand at 998.900 kb on - strand at 998.902 kb on + strand at 998.965 kb on + strand, within BPHYT_RS04340 at 999.212 kb on + strand, within BPHYT_RS04340 at 999.343 kb on + strand, within BPHYT_RS04340 at 999.494 kb on + strand, within BPHYT_RS04340 at 999.556 kb on + strand, within BPHYT_RS04340 at 999.557 kb on - strand, within BPHYT_RS04340 at 999.848 kb on - strand, within BPHYT_RS04340 at 999.848 kb on - strand, within BPHYT_RS04340 at 1000.058 kb on - strand at 1001.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 998,117 + BPHYT_RS04330 0.79 -1.5 998,203 + BPHYT_RS04330 0.87 -0.9 998,203 + BPHYT_RS04330 0.87 -1.1 998,204 - BPHYT_RS04330 0.87 -1.4 998,215 - BPHYT_RS04330 0.88 -2.2 998,215 - BPHYT_RS04330 0.88 -0.1 998,528 + BPHYT_RS04335 0.28 +0.6 998,528 + BPHYT_RS04335 0.28 +0.0 998,529 - BPHYT_RS04335 0.28 +2.6 998,836 - -1.8 998,836 - -2.7 998,899 + +1.0 998,900 - -1.2 998,900 - +2.6 998,902 + -0.6 998,965 + BPHYT_RS04340 0.10 +1.6 999,212 + BPHYT_RS04340 0.30 -0.9 999,343 + BPHYT_RS04340 0.40 +1.6 999,494 + BPHYT_RS04340 0.52 -0.4 999,556 + BPHYT_RS04340 0.56 -0.7 999,557 - BPHYT_RS04340 0.56 +0.6 999,848 - BPHYT_RS04340 0.79 -0.6 999,848 - BPHYT_RS04340 0.79 +0.0 1,000,058 - +0.6 1,001,112 - -0.7
Or see this region's nucleotide sequence