Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03405 and BPHYT_RS03410 are separated by 162 nucleotides BPHYT_RS03410 and BPHYT_RS03415 are separated by 211 nucleotides BPHYT_RS03415 and BPHYT_RS03420 are separated by 118 nucleotides
BPHYT_RS03405: BPHYT_RS03405 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 767,829 to 768,887
_RS03405
BPHYT_RS03410: BPHYT_RS03410 - queuine tRNA-ribosyltransferase, at 769,050 to 770,264
_RS03410
BPHYT_RS03415: BPHYT_RS03415 - preprotein translocase subunit YajC, at 770,476 to 770,805
_RS03415
BPHYT_RS03420: BPHYT_RS03420 - preprotein translocase subunit SecD, at 770,924 to 772,987
_RS03420
Position (kb) 769
770
771 Strain fitness (log2 ratio) -2
-1
0
1 at 768.288 kb on + strand, within BPHYT_RS03405 at 768.523 kb on - strand, within BPHYT_RS03405 at 768.691 kb on + strand, within BPHYT_RS03405 at 768.810 kb on + strand at 768.811 kb on - strand at 768.811 kb on - strand at 768.849 kb on + strand at 768.849 kb on + strand at 768.905 kb on + strand at 768.959 kb on + strand at 768.960 kb on - strand at 769.586 kb on - strand, within BPHYT_RS03410 at 770.082 kb on + strand, within BPHYT_RS03410 at 770.104 kb on + strand, within BPHYT_RS03410 at 770.151 kb on + strand at 770.151 kb on + strand at 770.151 kb on + strand at 770.155 kb on - strand at 770.155 kb on - strand at 770.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 768,288 + BPHYT_RS03405 0.43 -0.3 768,523 - BPHYT_RS03405 0.66 +0.5 768,691 + BPHYT_RS03405 0.81 -1.4 768,810 + +0.5 768,811 - -0.4 768,811 - -0.2 768,849 + +0.5 768,849 + -0.3 768,905 + +0.4 768,959 + -2.5 768,960 - -1.5 769,586 - BPHYT_RS03410 0.44 -0.2 770,082 + BPHYT_RS03410 0.85 -0.1 770,104 + BPHYT_RS03410 0.87 -2.0 770,151 + +1.6 770,151 + -0.7 770,151 + -2.3 770,155 - +1.2 770,155 - -1.5 770,155 - +0.0
Or see this region's nucleotide sequence