Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02565 and BPHYT_RS02570 are separated by 96 nucleotides BPHYT_RS02570 and BPHYT_RS02575 are separated by 305 nucleotides BPHYT_RS02575 and BPHYT_RS02580 are separated by 87 nucleotides
BPHYT_RS02565: BPHYT_RS02565 - PTS mannose transporter subunit IIA, at 582,866 to 583,393
_RS02565
BPHYT_RS02570: BPHYT_RS02570 - phosphocarrier protein HPr, at 583,490 to 583,759
_RS02570
BPHYT_RS02575: BPHYT_RS02575 - phosphoenolpyruvate-protein phosphotransferase, at 584,065 to 585,813
_RS02575
BPHYT_RS02580: BPHYT_RS02580 - molybdopterin biosynthesis protein MoeB, at 585,901 to 586,659
_RS02580
Position (kb)
584
585
586 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 583.389 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.392 kb on - strand at 583.392 kb on - strand at 583.449 kb on + strand at 583.468 kb on + strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.574 kb on + strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.994 kb on + strand at 583.995 kb on - strand at 584.051 kb on + strand at 584.052 kb on - strand at 584.052 kb on - strand at 584.150 kb on + strand at 584.158 kb on + strand at 584.158 kb on + strand at 584.159 kb on - strand at 584.227 kb on + strand at 584.437 kb on + strand, within BPHYT_RS02575 at 584.736 kb on + strand, within BPHYT_RS02575 at 584.797 kb on + strand, within BPHYT_RS02575 at 584.798 kb on - strand, within BPHYT_RS02575 at 584.798 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.994 kb on + strand, within BPHYT_RS02575 at 584.995 kb on - strand, within BPHYT_RS02575 at 584.995 kb on - strand, within BPHYT_RS02575 at 585.355 kb on + strand, within BPHYT_RS02575 at 585.355 kb on + strand, within BPHYT_RS02575 at 585.473 kb on - strand, within BPHYT_RS02575 at 585.517 kb on + strand, within BPHYT_RS02575 at 585.517 kb on + strand, within BPHYT_RS02575 at 585.871 kb on + strand at 585.871 kb on + strand at 585.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 583,389 + +2.7 583,391 + +2.9 583,391 + -0.9 583,391 + +1.7 583,392 - -0.4 583,392 - -0.4 583,449 + +0.5 583,468 + -0.5 583,511 - -1.5 583,511 - -2.5 583,511 - -1.8 583,574 + BPHYT_RS02570 0.31 -1.8 583,575 - BPHYT_RS02570 0.31 -1.4 583,575 - BPHYT_RS02570 0.31 -1.2 583,994 + +0.8 583,995 - +0.5 584,051 + +2.1 584,052 - -1.2 584,052 - -1.3 584,150 + -2.4 584,158 + -1.3 584,158 + -2.3 584,159 - -1.1 584,227 + -1.3 584,437 + BPHYT_RS02575 0.21 -2.5 584,736 + BPHYT_RS02575 0.38 -1.9 584,797 + BPHYT_RS02575 0.42 -1.6 584,798 - BPHYT_RS02575 0.42 -0.8 584,798 - BPHYT_RS02575 0.42 -1.7 584,836 - BPHYT_RS02575 0.44 -0.6 584,836 - BPHYT_RS02575 0.44 -1.8 584,836 - BPHYT_RS02575 0.44 -2.0 584,994 + BPHYT_RS02575 0.53 -1.1 584,995 - BPHYT_RS02575 0.53 -1.2 584,995 - BPHYT_RS02575 0.53 -1.0 585,355 + BPHYT_RS02575 0.74 -1.4 585,355 + BPHYT_RS02575 0.74 -1.5 585,473 - BPHYT_RS02575 0.81 -2.1 585,517 + BPHYT_RS02575 0.83 -2.4 585,517 + BPHYT_RS02575 0.83 +0.0 585,871 + +0.3 585,871 + -1.8 585,872 - +0.3
Or see this region's nucleotide sequence