Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01820 and BPHYT_RS01825 are separated by 371 nucleotides BPHYT_RS01825 and BPHYT_RS01830 are separated by 113 nucleotides
BPHYT_RS01820: BPHYT_RS01820 - sugar ABC transporter ATP-binding protein, at 412,723 to 414,357
_RS01820
BPHYT_RS01825: BPHYT_RS01825 - sugar ABC transporter substrate-binding protein, at 414,729 to 415,748
_RS01825
BPHYT_RS01830: BPHYT_RS01830 - LacI family transcriptional regulator, at 415,862 to 416,914
_RS01830
Position (kb) 414
415
416 Strain fitness (log2 ratio) -2
-1
0
1
2 at 414.063 kb on - strand, within BPHYT_RS01820 at 414.063 kb on - strand, within BPHYT_RS01820 at 414.241 kb on + strand at 414.242 kb on - strand at 414.318 kb on + strand at 414.473 kb on + strand at 414.474 kb on - strand at 414.483 kb on - strand at 414.609 kb on - strand at 414.609 kb on - strand at 414.609 kb on - strand at 414.667 kb on - strand at 414.699 kb on + strand at 414.739 kb on + strand at 414.773 kb on - strand at 415.294 kb on + strand, within BPHYT_RS01825 at 415.294 kb on + strand, within BPHYT_RS01825 at 415.295 kb on - strand, within BPHYT_RS01825 at 415.295 kb on - strand, within BPHYT_RS01825 at 415.416 kb on + strand, within BPHYT_RS01825 at 415.762 kb on - strand at 415.780 kb on - strand at 415.780 kb on - strand at 415.780 kb on - strand at 415.789 kb on + strand at 415.794 kb on + strand at 415.795 kb on - strand at 415.838 kb on + strand at 415.874 kb on - strand at 416.368 kb on + strand, within BPHYT_RS01830 at 416.368 kb on + strand, within BPHYT_RS01830 at 416.368 kb on + strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.369 kb on - strand, within BPHYT_RS01830 at 416.370 kb on + strand, within BPHYT_RS01830 at 416.370 kb on + strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.371 kb on - strand, within BPHYT_RS01830 at 416.471 kb on - strand at 416.472 kb on + strand, within BPHYT_RS01830 at 416.472 kb on + strand, within BPHYT_RS01830 at 416.472 kb on + strand, within BPHYT_RS01830 at 416.491 kb on + strand, within BPHYT_RS01830 at 416.492 kb on - strand, within BPHYT_RS01830 at 416.536 kb on - strand, within BPHYT_RS01830 at 416.628 kb on + strand, within BPHYT_RS01830 at 416.628 kb on + strand, within BPHYT_RS01830 at 416.656 kb on - strand, within BPHYT_RS01830 at 416.724 kb on + strand, within BPHYT_RS01830 at 416.724 kb on + strand, within BPHYT_RS01830
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 414,063 - BPHYT_RS01820 0.82 -2.2 414,063 - BPHYT_RS01820 0.82 -0.7 414,241 + +0.5 414,242 - -0.2 414,318 + +0.5 414,473 + -0.2 414,474 - -0.5 414,483 - +0.9 414,609 - -0.6 414,609 - +1.9 414,609 - -1.3 414,667 - +0.2 414,699 + +0.3 414,739 + -0.8 414,773 - +1.0 415,294 + BPHYT_RS01825 0.55 -2.6 415,294 + BPHYT_RS01825 0.55 -1.0 415,295 - BPHYT_RS01825 0.55 +0.3 415,295 - BPHYT_RS01825 0.55 -1.3 415,416 + BPHYT_RS01825 0.67 -0.1 415,762 - -1.3 415,780 - -1.5 415,780 - -0.4 415,780 - -0.9 415,789 + -0.4 415,794 + +0.7 415,795 - +0.4 415,838 + +1.5 415,874 - +0.7 416,368 + BPHYT_RS01830 0.48 +1.2 416,368 + BPHYT_RS01830 0.48 -0.3 416,368 + BPHYT_RS01830 0.48 -0.8 416,369 - BPHYT_RS01830 0.48 +0.3 416,369 - BPHYT_RS01830 0.48 -0.0 416,369 - BPHYT_RS01830 0.48 +0.6 416,370 + BPHYT_RS01830 0.48 +0.1 416,370 + BPHYT_RS01830 0.48 +0.4 416,371 - BPHYT_RS01830 0.48 -1.2 416,371 - BPHYT_RS01830 0.48 +0.5 416,371 - BPHYT_RS01830 0.48 +0.2 416,371 - BPHYT_RS01830 0.48 +0.1 416,471 - -0.9 416,472 + BPHYT_RS01830 0.58 -0.8 416,472 + BPHYT_RS01830 0.58 +1.2 416,472 + BPHYT_RS01830 0.58 -0.0 416,491 + BPHYT_RS01830 0.60 -0.3 416,492 - BPHYT_RS01830 0.60 -1.2 416,536 - BPHYT_RS01830 0.64 -1.4 416,628 + BPHYT_RS01830 0.73 -0.0 416,628 + BPHYT_RS01830 0.73 -1.3 416,656 - BPHYT_RS01830 0.75 +0.8 416,724 + BPHYT_RS01830 0.82 -0.9 416,724 + BPHYT_RS01830 0.82 +0.4
Or see this region's nucleotide sequence