Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00645 and BPHYT_RS00650 overlap by 4 nucleotides BPHYT_RS00650 and BPHYT_RS00655 are separated by 25 nucleotides BPHYT_RS00655 and BPHYT_RS00660 are separated by 2 nucleotides
BPHYT_RS00645: BPHYT_RS00645 - diaminopimelate decarboxylase, at 152,710 to 153,918
_RS00645
BPHYT_RS00650: BPHYT_RS00650 - acyl-CoA ligase, at 153,915 to 155,534
_RS00650
BPHYT_RS00655: BPHYT_RS00655 - 3-oxoacyl-ACP synthase, at 155,560 to 156,507
_RS00655
BPHYT_RS00660: BPHYT_RS00660 - 3-oxoacyl-ACP synthase, at 156,510 to 157,532
_RS00660
Position (kb) 153
154
155
156 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.068 kb on - strand, within BPHYT_RS00645 at 153.074 kb on + strand, within BPHYT_RS00645 at 153.075 kb on - strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.230 kb on - strand, within BPHYT_RS00645 at 153.707 kb on + strand, within BPHYT_RS00645 at 153.819 kb on - strand at 153.870 kb on + strand at 154.030 kb on + strand at 154.040 kb on + strand at 154.057 kb on + strand at 154.057 kb on + strand at 154.057 kb on + strand at 154.058 kb on - strand at 154.058 kb on - strand at 154.128 kb on + strand, within BPHYT_RS00650 at 154.128 kb on + strand, within BPHYT_RS00650 at 154.129 kb on - strand, within BPHYT_RS00650 at 154.279 kb on + strand, within BPHYT_RS00650 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.592 kb on - strand, within BPHYT_RS00650 at 154.667 kb on + strand, within BPHYT_RS00650 at 154.668 kb on - strand, within BPHYT_RS00650 at 154.678 kb on + strand, within BPHYT_RS00650 at 154.722 kb on - strand, within BPHYT_RS00650 at 155.007 kb on - strand, within BPHYT_RS00650 at 155.007 kb on - strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.008 kb on + strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.009 kb on - strand, within BPHYT_RS00650 at 155.446 kb on + strand at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.711 kb on + strand, within BPHYT_RS00655 at 155.858 kb on + strand, within BPHYT_RS00655 at 155.859 kb on - strand, within BPHYT_RS00655 at 155.859 kb on - strand, within BPHYT_RS00655 at 155.909 kb on + strand, within BPHYT_RS00655 at 155.924 kb on + strand, within BPHYT_RS00655 at 156.268 kb on - strand, within BPHYT_RS00655 at 156.512 kb on - strand at 156.523 kb on + strand at 156.524 kb on - strand at 156.524 kb on - strand at 156.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 153,050 + BPHYT_RS00645 0.28 -1.0 153,050 + BPHYT_RS00645 0.28 -0.9 153,050 + BPHYT_RS00645 0.28 -0.3 153,050 + BPHYT_RS00645 0.28 +0.2 153,050 + BPHYT_RS00645 0.28 -0.1 153,050 + BPHYT_RS00645 0.28 -0.7 153,050 + BPHYT_RS00645 0.28 -1.9 153,051 - BPHYT_RS00645 0.28 -0.3 153,051 - BPHYT_RS00645 0.28 +0.1 153,068 - BPHYT_RS00645 0.30 -0.4 153,074 + BPHYT_RS00645 0.30 -1.1 153,075 - BPHYT_RS00645 0.30 +0.3 153,229 + BPHYT_RS00645 0.43 -2.3 153,229 + BPHYT_RS00645 0.43 +0.1 153,230 - BPHYT_RS00645 0.43 -0.6 153,707 + BPHYT_RS00645 0.82 +0.7 153,819 - -0.4 153,870 + +4.7 154,030 + -2.1 154,040 + -0.4 154,057 + -0.8 154,057 + -0.6 154,057 + +0.9 154,058 - -1.6 154,058 - +0.3 154,128 + BPHYT_RS00650 0.13 -0.5 154,128 + BPHYT_RS00650 0.13 -1.1 154,129 - BPHYT_RS00650 0.13 -0.9 154,279 + BPHYT_RS00650 0.22 -1.2 154,591 + BPHYT_RS00650 0.42 +0.2 154,591 + BPHYT_RS00650 0.42 -1.7 154,592 - BPHYT_RS00650 0.42 -0.6 154,667 + BPHYT_RS00650 0.46 -0.8 154,668 - BPHYT_RS00650 0.46 +0.1 154,678 + BPHYT_RS00650 0.47 -0.1 154,722 - BPHYT_RS00650 0.50 -0.4 155,007 - BPHYT_RS00650 0.67 -0.2 155,007 - BPHYT_RS00650 0.67 -1.1 155,008 + BPHYT_RS00650 0.67 -2.0 155,008 + BPHYT_RS00650 0.67 -0.9 155,008 + BPHYT_RS00650 0.67 -1.9 155,008 + BPHYT_RS00650 0.67 +0.4 155,009 - BPHYT_RS00650 0.68 +1.3 155,009 - BPHYT_RS00650 0.68 +0.9 155,009 - BPHYT_RS00650 0.68 +0.1 155,446 + -0.4 155,711 + BPHYT_RS00655 0.16 +5.0 155,711 + BPHYT_RS00655 0.16 -1.3 155,711 + BPHYT_RS00655 0.16 +1.1 155,711 + BPHYT_RS00655 0.16 -0.0 155,711 + BPHYT_RS00655 0.16 -1.1 155,711 + BPHYT_RS00655 0.16 +0.3 155,711 + BPHYT_RS00655 0.16 -0.5 155,711 + BPHYT_RS00655 0.16 +0.4 155,858 + BPHYT_RS00655 0.31 -1.1 155,859 - BPHYT_RS00655 0.32 -0.7 155,859 - BPHYT_RS00655 0.32 -1.2 155,909 + BPHYT_RS00655 0.37 +0.6 155,924 + BPHYT_RS00655 0.38 +0.1 156,268 - BPHYT_RS00655 0.75 -0.6 156,512 - -0.4 156,523 + -0.9 156,524 - +1.4 156,524 - +1.1 156,524 - +0.0
Or see this region's nucleotide sequence