Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32365

Experiment: 24hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32360 and BPHYT_RS32365 are separated by 72 nucleotidesBPHYT_RS32365 and BPHYT_RS32370 are separated by 323 nucleotides BPHYT_RS32360: BPHYT_RS32360 - spermidine/putrescine ABC transporter ATPase, at 2,831,032 to 2,832,120 _RS32360 BPHYT_RS32365: BPHYT_RS32365 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,832,193 to 2,833,260 _RS32365 BPHYT_RS32370: BPHYT_RS32370 - LysR family transcriptional regulator, at 2,833,584 to 2,834,501 _RS32370 Position (kb) 2832 2833 2834Strain fitness (log2 ratio) -1 0 1 2 3 4at 2831.339 kb on - strand, within BPHYT_RS32360at 2832.096 kb on + strandat 2832.141 kb on - strandat 2832.402 kb on - strand, within BPHYT_RS32365at 2832.503 kb on - strand, within BPHYT_RS32365at 2832.880 kb on + strand, within BPHYT_RS32365at 2832.880 kb on + strand, within BPHYT_RS32365at 2832.897 kb on - strand, within BPHYT_RS32365at 2833.227 kb on + strandat 2833.380 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.488 kb on - strandat 2833.489 kb on + strandat 2833.489 kb on + strandat 2833.490 kb on - strandat 2833.496 kb on + strandat 2833.788 kb on + strand, within BPHYT_RS32370at 2833.921 kb on + strandat 2834.032 kb on + strand, within BPHYT_RS32370at 2834.036 kb on + strand, within BPHYT_RS32370at 2834.036 kb on + strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.057 kb on + strand, within BPHYT_RS32370

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1
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2,831,339 - BPHYT_RS32360 0.28 +1.6
2,832,096 + -0.3
2,832,141 - +0.2
2,832,402 - BPHYT_RS32365 0.20 +1.2
2,832,503 - BPHYT_RS32365 0.29 +4.2
2,832,880 + BPHYT_RS32365 0.64 +0.7
2,832,880 + BPHYT_RS32365 0.64 +0.3
2,832,897 - BPHYT_RS32365 0.66 +1.1
2,833,227 + +0.1
2,833,380 + -0.6
2,833,487 + +0.7
2,833,487 + -0.7
2,833,487 + -0.5
2,833,487 + +2.8
2,833,487 + +0.7
2,833,488 - +1.0
2,833,489 + -0.5
2,833,489 + +0.6
2,833,490 - -0.8
2,833,496 + +0.6
2,833,788 + BPHYT_RS32370 0.22 -0.3
2,833,921 + +1.2
2,834,032 + BPHYT_RS32370 0.49 -0.4
2,834,036 + BPHYT_RS32370 0.49 -0.4
2,834,036 + BPHYT_RS32370 0.49 +0.8
2,834,056 - BPHYT_RS32370 0.51 +1.8
2,834,056 - BPHYT_RS32370 0.51 +0.6
2,834,056 - BPHYT_RS32370 0.51 +2.0
2,834,057 + BPHYT_RS32370 0.52 -1.2

Or see this region's nucleotide sequence