Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31410 and BPHYT_RS31415 are separated by 23 nucleotides BPHYT_RS31415 and BPHYT_RS31420 are separated by 54 nucleotides BPHYT_RS31420 and BPHYT_RS31425 are separated by 33 nucleotides BPHYT_RS31425 and BPHYT_RS31430 are separated by 52 nucleotides
BPHYT_RS31410: BPHYT_RS31410 - D-amino acid oxidase, at 2,601,630 to 2,602,970
_RS31410
BPHYT_RS31415: BPHYT_RS31415 - amino acid ABC transporter permease, at 2,602,994 to 2,603,791
_RS31415
BPHYT_RS31420: BPHYT_RS31420 - ABC transporter substrate-binding protein, at 2,603,846 to 2,604,658
_RS31420
BPHYT_RS31425: BPHYT_RS31425 - arginine ABC transporter ATP-binding protein, at 2,604,692 to 2,605,393
_RS31425
BPHYT_RS31430: BPHYT_RS31430 - FAD-dependent oxidoreductase, at 2,605,446 to 2,606,771
_RS31430
Position (kb) 2603
2604
2605 Strain fitness (log2 ratio) -1
0
1
2 at 2602.881 kb on + strand at 2603.272 kb on - strand, within BPHYT_RS31415 at 2604.181 kb on - strand, within BPHYT_RS31420 at 2604.650 kb on + strand at 2604.996 kb on + strand, within BPHYT_RS31425 at 2605.363 kb on - strand at 2605.428 kb on - strand at 2605.602 kb on - strand, within BPHYT_RS31430
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 2,602,881 + -0.8 2,603,272 - BPHYT_RS31415 0.35 -0.9 2,604,181 - BPHYT_RS31420 0.41 +2.2 2,604,650 + +0.9 2,604,996 + BPHYT_RS31425 0.43 -1.2 2,605,363 - -0.1 2,605,428 - +1.5 2,605,602 - BPHYT_RS31430 0.12 +0.3
Or see this region's nucleotide sequence