Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotides BPHYT_RS24175 and BPHYT_RS24180 are separated by 153 nucleotides BPHYT_RS24180 and BPHYT_RS24185 are separated by 7 nucleotides
BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737
_RS24170
BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317
_RS24175
BPHYT_RS24180: BPHYT_RS24180 - C4-dicarboxylate ABC transporter substrate-binding protein, at 993,471 to 993,950
_RS24180
BPHYT_RS24185: BPHYT_RS24185 - C4-dicarboxylate ABC transporter permease, at 993,958 to 995,310
_RS24185
Position (kb) 992
993
994 Strain fitness (log2 ratio) -2
-1
0
1
2 at 991.342 kb on + strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.994 kb on - strand at 992.040 kb on + strand at 992.330 kb on + strand at 992.331 kb on - strand at 992.446 kb on - strand, within BPHYT_RS24175 at 992.531 kb on + strand, within BPHYT_RS24175 at 992.784 kb on + strand, within BPHYT_RS24175 at 992.917 kb on - strand, within BPHYT_RS24175
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 991,342 + BPHYT_RS24170 0.57 -1.5 991,343 - BPHYT_RS24170 0.58 +0.8 991,343 - BPHYT_RS24170 0.58 +0.3 991,994 - -2.5 992,040 + +2.2 992,330 + -0.7 992,331 - -0.1 992,446 - BPHYT_RS24175 0.12 -0.0 992,531 + BPHYT_RS24175 0.21 -0.6 992,784 + BPHYT_RS24175 0.46 -2.3 992,917 - BPHYT_RS24175 0.60 -0.3
Or see this region's nucleotide sequence