Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23460 and BPHYT_RS23465 are separated by 213 nucleotides BPHYT_RS23465 and BPHYT_RS23470 are separated by 89 nucleotides BPHYT_RS23470 and BPHYT_RS23475 are separated by 72 nucleotides
BPHYT_RS23460: BPHYT_RS23460 - AraC family transcriptional regulator, at 831,153 to 832,109
_RS23460
BPHYT_RS23465: BPHYT_RS23465 - aliphatic nitrilase, at 832,323 to 833,309
_RS23465
BPHYT_RS23470: BPHYT_RS23470 - carbonic anhydrase, at 833,399 to 833,953
_RS23470
BPHYT_RS23475: BPHYT_RS23475 - luciferase, at 834,026 to 835,033
_RS23475
Position (kb) 832
833
834 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 832.110 kb on - strand at 832.110 kb on - strand at 832.253 kb on + strand at 832.371 kb on + strand at 832.372 kb on - strand at 832.470 kb on + strand, within BPHYT_RS23465 at 832.471 kb on - strand, within BPHYT_RS23465 at 832.471 kb on - strand, within BPHYT_RS23465 at 832.648 kb on - strand, within BPHYT_RS23465 at 833.015 kb on + strand, within BPHYT_RS23465 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.447 kb on - strand at 833.488 kb on + strand, within BPHYT_RS23470 at 833.714 kb on - strand, within BPHYT_RS23470 at 834.084 kb on + strand at 834.177 kb on - strand, within BPHYT_RS23475 at 834.202 kb on + strand, within BPHYT_RS23475
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 832,110 - +0.2 832,110 - +1.1 832,253 + +0.3 832,371 + -0.6 832,372 - -0.0 832,470 + BPHYT_RS23465 0.15 -0.2 832,471 - BPHYT_RS23465 0.15 +1.3 832,471 - BPHYT_RS23465 0.15 -0.9 832,648 - BPHYT_RS23465 0.33 -1.0 833,015 + BPHYT_RS23465 0.70 +0.6 833,085 + BPHYT_RS23465 0.77 -0.3 833,085 + BPHYT_RS23465 0.77 -0.5 833,447 - -2.9 833,488 + BPHYT_RS23470 0.16 +1.4 833,714 - BPHYT_RS23470 0.57 +0.9 834,084 + +0.7 834,177 - BPHYT_RS23475 0.15 -0.2 834,202 + BPHYT_RS23475 0.17 -0.8
Or see this region's nucleotide sequence