Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23030 and BPHYT_RS23035 are separated by 367 nucleotides BPHYT_RS23035 and BPHYT_RS23040 are separated by 26 nucleotides
BPHYT_RS23030: BPHYT_RS23030 - gluconate 2-dehydrogenase, at 727,734 to 728,474
_RS23030
BPHYT_RS23035: BPHYT_RS23035 - hypothetical protein, at 728,842 to 729,639
_RS23035
BPHYT_RS23040: BPHYT_RS23040 - peptidase, at 729,666 to 731,936
_RS23040
Position (kb) 728
729
730 Strain fitness (log2 ratio) -2
-1
0
1 at 728.143 kb on + strand, within BPHYT_RS23030 at 728.436 kb on + strand at 728.456 kb on + strand at 728.457 kb on - strand at 728.482 kb on + strand at 728.483 kb on - strand at 728.483 kb on - strand at 728.585 kb on - strand at 728.819 kb on + strand at 728.819 kb on + strand at 728.820 kb on - strand at 728.820 kb on - strand at 728.904 kb on + strand at 728.946 kb on - strand, within BPHYT_RS23035 at 729.277 kb on - strand, within BPHYT_RS23035 at 729.512 kb on + strand, within BPHYT_RS23035 at 729.745 kb on + strand at 729.746 kb on - strand at 729.794 kb on - strand at 729.830 kb on + strand at 729.952 kb on + strand, within BPHYT_RS23040 at 729.953 kb on - strand, within BPHYT_RS23040 at 729.953 kb on - strand, within BPHYT_RS23040 at 730.040 kb on - strand, within BPHYT_RS23040 at 730.077 kb on - strand, within BPHYT_RS23040 at 730.077 kb on - strand, within BPHYT_RS23040 at 730.079 kb on - strand, within BPHYT_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 728,143 + BPHYT_RS23030 0.55 -0.3 728,436 + +0.5 728,456 + -0.1 728,457 - -0.4 728,482 + -0.7 728,483 - +0.3 728,483 - +1.3 728,585 - -0.0 728,819 + +0.1 728,819 + -1.2 728,820 - +0.1 728,820 - -0.2 728,904 + -0.2 728,946 - BPHYT_RS23035 0.13 +1.6 729,277 - BPHYT_RS23035 0.55 -1.7 729,512 + BPHYT_RS23035 0.84 +1.4 729,745 + -0.2 729,746 - +0.5 729,794 - -2.1 729,830 + +0.5 729,952 + BPHYT_RS23040 0.13 -0.3 729,953 - BPHYT_RS23040 0.13 -0.5 729,953 - BPHYT_RS23040 0.13 +0.3 730,040 - BPHYT_RS23040 0.16 -0.0 730,077 - BPHYT_RS23040 0.18 -0.8 730,077 - BPHYT_RS23040 0.18 -0.3 730,079 - BPHYT_RS23040 0.18 -0.5
Or see this region's nucleotide sequence