Experiment: 24hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20750 and BPHYT_RS20755 are separated by 110 nucleotides BPHYT_RS20755 and BPHYT_RS20760 are separated by 251 nucleotides
BPHYT_RS20750: BPHYT_RS20750 - LacI family transcriptional regulator, at 216,418 to 217,356
_RS20750
BPHYT_RS20755: BPHYT_RS20755 - fuconate dehydratase, at 217,467 to 218,765
_RS20755
BPHYT_RS20760: BPHYT_RS20760 - oxidoreductase domain-containing protein, at 219,017 to 219,985
_RS20760
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 216.483 kb on - strand at 216.722 kb on + strand, within BPHYT_RS20750 at 216.986 kb on - strand, within BPHYT_RS20750 at 217.465 kb on - strand at 217.572 kb on + strand at 217.695 kb on - strand, within BPHYT_RS20755 at 218.545 kb on - strand, within BPHYT_RS20755 at 218.613 kb on + strand, within BPHYT_RS20755 at 218.670 kb on + strand at 218.672 kb on + strand at 218.673 kb on - strand at 218.773 kb on + strand at 218.815 kb on + strand at 218.937 kb on - strand at 219.027 kb on - strand at 219.027 kb on - strand at 219.074 kb on + strand at 219.074 kb on + strand at 219.074 kb on + strand at 219.211 kb on - strand, within BPHYT_RS20760 at 219.271 kb on + strand, within BPHYT_RS20760 at 219.272 kb on - strand, within BPHYT_RS20760 at 219.272 kb on - strand, within BPHYT_RS20760 at 219.517 kb on - strand, within BPHYT_RS20760 at 219.521 kb on - strand, within BPHYT_RS20760 at 219.582 kb on + strand, within BPHYT_RS20760 at 219.626 kb on + strand, within BPHYT_RS20760
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO11 MOI 0.1 remove 216,483 - +1.1 216,722 + BPHYT_RS20750 0.32 -2.9 216,986 - BPHYT_RS20750 0.60 +0.2 217,465 - +0.1 217,572 + -0.2 217,695 - BPHYT_RS20755 0.18 -1.1 218,545 - BPHYT_RS20755 0.83 -0.8 218,613 + BPHYT_RS20755 0.88 +0.6 218,670 + -1.1 218,672 + -0.6 218,673 - +1.0 218,773 + +3.7 218,815 + -1.0 218,937 - -0.7 219,027 - -0.1 219,027 - -1.9 219,074 + -0.4 219,074 + +0.4 219,074 + +0.5 219,211 - BPHYT_RS20760 0.20 +0.9 219,271 + BPHYT_RS20760 0.26 -0.7 219,272 - BPHYT_RS20760 0.26 -0.3 219,272 - BPHYT_RS20760 0.26 -1.4 219,517 - BPHYT_RS20760 0.52 -0.4 219,521 - BPHYT_RS20760 0.52 +0.4 219,582 + BPHYT_RS20760 0.58 -2.1 219,626 + BPHYT_RS20760 0.63 -0.1
Or see this region's nucleotide sequence